Hi mjulista ,
Welcome to science buddies!
I think you are doing this excellent, but challenging project.
https://www.sciencebuddies.org/science- ... p009.shtml
In this project you need to first identify a target tissue (organ type) that you would be interested in creating from another tissue. Next, you need to use the bioinformatics database to identify the transcription factors that are present in this issue. And finally, you need to find out what other tissues also produce the same transcription factors.
The idea is to identify a tissue that produces a high percentage of the transcription factors that are needed for your target tissue, as this will probably be a good candidate for transforming to the target tissue. The example shown in the project is for kidney cells.
The columns on your table should be as shown in the table in the procedure section, first column, "target cell type." second column "important transcription factors, third column "other cell types that express it," : fourth column "highly expressed in other cell types." and fifth column, "known functions." . You pick the target cell type and then find the important transcription factors, and information for the other columns in the database.
Does this help? What target cell type are you planning to transform?
Donna Hardy