Soil-Based Microbial Fuel Cells

Ask questions about projects relating to: biology, biochemistry, genomics, microbiology, molecular biology, pharmacology/toxicology, zoology, human behavior, archeology, anthropology, political science, sociology, geology, environmental science, oceanography, seismology, weather, or atmosphere.

Moderators: AmyCowen, kgudger, MadelineB, Moderators

Crazy_Mad_Scientist
Posts: 71
Joined: Wed Sep 14, 2016 7:14 pm
Occupation: Student

Re: Soil-Based Microbial Fuel Cells

Post by Crazy_Mad_Scientist »

Hi Donna,

I just sent out the endorsement request to ScienceBuddies! Thank you so much!

My school has some electrolysis supplies, so I can ask to run a few trials with varied concentrations of acetic acid. Thank you for the idea!

Sincerely,
CMS
donnahardy2
Former Expert
Posts: 2671
Joined: Mon Nov 14, 2005 12:45 pm

Re: Soil-Based Microbial Fuel Cells

Post by donnahardy2 »

Hi CMS,

Thanks for letting me know. I have never received a response to my inquiry about how I can post an endorsement without violating our confidentiality policy, so I will send another inquiry. Hopefully we can make some progress this time.

It's great that you have electrolysis equipment. You can try a small experiment and verify that it works first.

Let me know how it goes.

Donna
Crazy_Mad_Scientist
Posts: 71
Joined: Wed Sep 14, 2016 7:14 pm
Occupation: Student

Re: Soil-Based Microbial Fuel Cells

Post by Crazy_Mad_Scientist »

Hi Donna,

Good news! My proposal finally got approved! Have you recieved the email for the endorsement from sciencebuddies?

Thank you!
CMS
donnahardy2
Former Expert
Posts: 2671
Joined: Mon Nov 14, 2005 12:45 pm

Re: Soil-Based Microbial Fuel Cells

Post by donnahardy2 »

Hi CMS,

Congratulations! This is great news.

Yes, I got the link, but I can't open it. I sent a message back asking for help in opening it, but I haven't gotten a response yet. . The link requires the application Outlook, which I don't have and can't seem to get installed on my computer.

Let me know if you have any ideas.

Donna
Crazy_Mad_Scientist
Posts: 71
Joined: Wed Sep 14, 2016 7:14 pm
Occupation: Student

Re: Soil-Based Microbial Fuel Cells

Post by Crazy_Mad_Scientist »

Hi Donna,

Hmm, I tried sending myself an endorsement request to see the problem. I think that Experiment may have updated the endorsement link, so it should not require Outlook anymore. I will try resending the link to sciencebuddies. After clicking on the link, it should take you directly to the proposal page with the endorsement link on top.

If that doesn't work, I have the link here: https://experiment.com/projects/8340/endorse/7AOLrDs. It should take you to the endorsement page. :D

Thank you!
CMS
donnahardy2
Former Expert
Posts: 2671
Joined: Mon Nov 14, 2005 12:45 pm

Re: Soil-Based Microbial Fuel Cells

Post by donnahardy2 »

Hi CMS,

I'm making progress and got into the page to submit the endorsement, but it requires a photo, which I don't have on my computer at the moment. I'll try to finish tomorrow or Monday. I'm so sorry for the delay.

Donna
Crazy_Mad_Scientist
Posts: 71
Joined: Wed Sep 14, 2016 7:14 pm
Occupation: Student

Re: Soil-Based Microbial Fuel Cells

Post by Crazy_Mad_Scientist »

That's alright.

CMS
Crazy_Mad_Scientist
Posts: 71
Joined: Wed Sep 14, 2016 7:14 pm
Occupation: Student

Re: Soil-Based Microbial Fuel Cells

Post by Crazy_Mad_Scientist »

Hi Donna,

If it is possible, can you post the endorsement by tonight? Tomorrow is supposed to be the launch date, but I need at least one endorsement to lauch :) .

Thank you so much!
CMS
Crazy_Mad_Scientist
Posts: 71
Joined: Wed Sep 14, 2016 7:14 pm
Occupation: Student

Re: Soil-Based Microbial Fuel Cells

Post by Crazy_Mad_Scientist »

Hi Donna,

I've been looking at an acetic acid-based electrolyte for electroplating. I found quite a lot of sources (although not research papers) on possible electrolyte solution for copper (not lead unfortunately).

Here is one forum which used vinegar (4% acetic acid in water), some hydrogen peroxide (3%), and a pinch of table salt:
http://www.finishing.com/500/90.shtml

This one is a bit more specific, and requires more materials:
https://www.google.com/patents/US2466660

I will probably not dilute my vinegar. For the hydrogen peroxide, will it be applicable to lead? It was added to oxidize copper, which would apparently spead up dissolution. I'm not quite sure if I should do this.

By the way I found this very useful research paper on mfc based recovery of four metals, including Pb2+. They used 2 mole HCl for the cathode electrolyte, and about 0.4 g of Pb/Litre: http://publications.lib.chalmers.se/rec ... 163014.pdf
To quantify the lead plated onto the cathode electrode, I will soak the electrode in vinegar for a few hours (around 5), and use the kit to determine lead concentration.

Thank you!
CMS
Crazy_Mad_Scientist
Posts: 71
Joined: Wed Sep 14, 2016 7:14 pm
Occupation: Student

Re: Soil-Based Microbial Fuel Cells

Post by Crazy_Mad_Scientist »

Hi Donna,

Is it possible to post the endorsment tonight? I need it to launch the project :D .

Thank you so much!
CMS
Crazy_Mad_Scientist
Posts: 71
Joined: Wed Sep 14, 2016 7:14 pm
Occupation: Student

Re: Soil-Based Microbial Fuel Cells

Post by Crazy_Mad_Scientist »

Hi Donna,

I ordered all of my arduino incubator supplies and the carbon cloth about a week ago! :D They should arrive soon. I have also finished programming and compiling the arduino code in preparation for the actual hardware design. I will be constructing my MFC later on the afternoon (However without the soil sample yet since it is raining.) I just want to let you know some of the research I've been doing on electrolytes in electrolysis.

The purpose of an electrolyte is to provide the ions (in this case Pb2+) and a conductive medium; the more conductive the better. Many studies simply used acids like HCL: http://publications.lib.chalmers.se/rec ... 163014.pdf.

I would use HCL if I wasn't using plastic containers to hold it... :( (Here are the containers I am using: https://ziploc.com/en/products/containe ... loc-medium).

Image

I do not want to risk drilling through a glass jar. Doing some further research I think I understand why vinegar and citric acid are not particularly used for electrolysis. Their conductivities are fairly low because of the lack of free ions in the solution. I did consider using table salt, but I realized that this would create chlorine gas, which is apparently dangerous. This sciencebuddies idea suggests magnesium sulfate (epsom salts) for the electrolyte solution since it is considerably conductive: https://www.sciencebuddies.org/science- ... background

This science fair project also used magnesium sulfate and explained some of the reactions if the magnesium were to be electrolysed (not something I would want). Surprisingly the voltages used were extremely high, so I may just get a tiny bit of the mangesium sulfate plated along with the reduced Pb2+. The tiny amount from the magnesium sulfate would mostly be magnesium hydroxide (which should not be poisonous): http://www.odec.ca/projects/2013/beso13 ... sults.html

IMPORTANT:
I do however have a problem with the MFC electrodes. I need to use conductive nickel epoxy or other conductive glues to attach my copper wire to the carbon cloth (I am still waiting for the cloth to be shipped though). I cannot find conductive glue anywhere, and purchasing them is quite expensive ($25.00 for 0.2 mL not including shipping). I also don't want to wait for the shipping, which should take around 4-5 weeks from Hong Kong or China. I have been looking at some diy conductive glue recipe using contact cement and graphite powder. Should I try this? The results are apparently pretty good.

BY THE WAY PLEASE READ ALL THE POSTS BEFORE THIS! IF YOU HAVE NOT READ THIS PAPER : http://publications.lib.chalmers.se/rec ... 163014.pdf, PLEASE DO. IT IS ALMOST 99% RELEVANT TO MY TOPIC.

Thank you!
CMS
Crazy_Mad_Scientist
Posts: 71
Joined: Wed Sep 14, 2016 7:14 pm
Occupation: Student

Re: Soil-Based Microbial Fuel Cells

Post by Crazy_Mad_Scientist »

Hi Donna,

I am still having trouble with my cathode electrolyte... Is it possible for you to explain this chemical reaction:

http://www.odec.ca/projects/2013/beso13 ... sults.html

The equations are on the top of the page. I just want to make sure any bi-products of electrolysing magnesium sulfate will not be poisonous.

Thank you!
CMS
donnahardy2
Former Expert
Posts: 2671
Joined: Mon Nov 14, 2005 12:45 pm

Re: Soil-Based Microbial Fuel Cells

Post by donnahardy2 »

Hi CMS,

I apologize for the delay; I had to switch to a new computer and I missed a couple of notifications from you. I should be able to keep up now.

I will answer your last post and go back and review the post from earlier this week and reply again tomorrow.

The equation you posted is for the reaction of the element magnesium in water. Magnesium is a very reactive element and will react with water to produce hydrogen gas. The end products are not hazardous; the elements are magnesium, sulfur, oxygen and hydrogen and magnesium sulfate is not particularly hazardous. However, the reaction of the element magnesium with air or water is quick and exothermic, so hopefully you are not planning to do this at home.

The reaction in the cathode electrode is different. It involves transferring the electrons generated in the anode chamber to an electrode acceptor and the reaction is not exothermic. The cathode reaction is required to complete the circuit of the MFC and generate electricity.

Have you set up the MFC yet?

Donna
donnahardy2
Former Expert
Posts: 2671
Joined: Mon Nov 14, 2005 12:45 pm

Re: Soil-Based Microbial Fuel Cells

Post by donnahardy2 »

Hi CMS,

You are really good at background research. The research paper on metal recovery is completely relevant to your project. It specifies the voltage that will work for recovery of Pb from the cathode electrode, so if your MFC will generate the voltage, you should be able to plate the Pb on the cathode electrode. The authors used 2 M HCl and I think it would be much safer for you to the the acetic acid mixture. At this point, you are not optimizing the system for maximum recovery; you just need to show that it will work.

The cost of the conductive glue is prohibitive, so you should definitely try the graphite alternative. Make sure you get the wire in contact with the carbon cloth

Donna
Crazy_Mad_Scientist
Posts: 71
Joined: Wed Sep 14, 2016 7:14 pm
Occupation: Student

Re: Soil-Based Microbial Fuel Cells

Post by Crazy_Mad_Scientist »

Hi Donna,

I bought my aquarium pump, some epoxies and tubing. I will still need to find a pipe to finish building the mfc, however.

For the electrolyte solution, I will probably be using this solution: http://forums.sohc4.net/index.php?topic=119931.0.

I don't want to use table salt because it will create chlorine gas. The magnesium sulfate will not be plated on the cathode (hopefully, since it requires a high Voltage?). It will set a good conductivity in the solution.

Here's a quick question: Since oxygen will not be the electron acceptor, will hydrogen gas accumulate in the cathode? Should I attach a tube to get rid of the excess gas?

Thank you so much!
CMS
Last edited by Crazy_Mad_Scientist on Tue Jan 03, 2017 10:58 pm, edited 1 time in total.
Locked

Return to “Grades 9-12: Life, Earth, and Social Sciences”