Biochemistry

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thilker
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Joined: Tue Mar 28, 2017 2:50 pm
Occupation: Student

Biochemistry

Post by thilker »

For my Science project I need to answer the following questions:

1. Which of the following is NOT an electron acceptor in the mitochondrial respiratory chain?
a)Fe3+
b)FMN
c)FAD
d)Cu2+
e)CoQH2

2. Which of the following statements about oxidative phosphorylation by ATP synthase is FALSE?
a)ATP synthesis occurs via simultaneous conformational changes in protein subunits containing catalytic sites.
b)A proton gradient alone without a corresponding energy input is not sufficient to drive the synthesis of ATP.
c)The electrochemical gradient across the inner mitochondrial membrane generated by electron flow is used to synthesize ATP.
d)Inhibitors disrupt electron flow as well as ATP synthesis.
e)Uncouplers dissipate the electrochemical gradient but electron transport continues without ATP production.

3.Which of the following is NOT true of respiratory control?
a) Oxidative phosphorylation is absolutely dependent on the continued flow of electrons from substrates to oxygen.
b) The stimulation of respiration by addition of ADP is stoichiometric.
c) The rate of respiration is controlled by the balance between the ΔG's for phosphorylation of ADP, electron transport and proton pumping.
d) Oxidative phosphorylation is regulated by allosteric mechanisms.
e) Maintenance of respiratory control depends on the structural integrity of the mitochondrial inner membrane.

Which of the following are part of the chemiosmotic coupling model?

1) Protons are pumped out of the mitochondrial matrix into the intermembrane space.
2) The active transport of protons is driven by the free energy derived from electron transport.
3) Increasing the concentration of protons on the other side of the inner membrane creates a charge and a pH imbalance that releases energy upon proton flow back into the matrix.
4) The energy released by the return of protons to the matrix is what drives ATP synthesis.
a) Only statements 2, 3, and 4 are correct.
b) Only statements 1, 2, and 3 are correct.
c) Only statements 1, 2, and 4 are correct.
d) All of the listed choices are correct.

Which statement about uncouplers is NOT true?
a) They allow electron transport without ATP synthesis.
b) They allow ATP synthesis with no electron transport.
c) 2,4-DNP is an example of an uncoupler.
d) Their mechanism of action is basically to transport protons back into the matrix, preventing the required buildup of charge on the other side.

The ATP yield from NADH transported across the mitochondrial inner membrane by the glycerol phosphate shuttle is the same as if the malate/aspartate shuttle were used.
a) True
b) False
MadelineB
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Re: Biochemistry

Post by MadelineB »

Hello Thilker and welcome to Science Buddies,

Please tell us more about your science project - what are your hypotheses and describe your experimental design for collecting the data to test those hypotheses. Tell us how you list of questions relate to your project, then the experts can help you find the answers to your questions.
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