Welcome to Science Buddies! I think you are doing this very excellent project from this website:http://www.sciencebuddies.org/science-f ... p017.shtml
The Bunsen burner and ethanol are used to sterilize inoculating loops and glass rods used in the sterile transfer of the bacteria. A Bunsen burner is used to sterilize an inoculating loop directly in the flame until the metal loop glows. For a glass rod, the rod is dipped in ethanol briefly and then exposed to the flame to sterilize it by burning off the ethanol.
In this project you will be transferring the bacterial culture from an agar slant to a Petri dish to streak it out so you can select a single colony to transfer to the broth culture that will be used to grow the broth culture.
The microbiological techniques section of the Science Buddies website is excellent, and I recommend reading through all of the sections so you can become familiar with the basic techniques. http://www.sciencebuddies.org/science-f ... ques.shtml
You can use sterile cotton swabs instead of an inoculating loop and glass rods, and eliminate the need for the Bunsen burner and ethanol. You can use 70% isopropanol available from the Dollar or drugstore instead of ethanol. Let me know if you need any other suggestions for substitutions.
One important consideration in doing a science project is to keep all parameters controlled, except the one independent variable that you are changing in the experiment, which in this experiment, is the time of UV exposure. So you will want to make sure that the bacteria you use are at the same growth stage at the time of inoculation. Probably the best way to do this is to transfer a single colony to a broth culture and incubate it overnight (or for a certain number of hours) until it is transferred to the Petri dish immediately prior to the UV step.
Here is information of one of many examples of a suitable UV face shield. The specifications state that it filters out the full UV spectrum, 200-405 nm. This is an expensive item, so hopefully you can borrow something suitable. If you do borrow something, check to make sure that the screen filters out all of the harmful UV rays. http://www.fishersci.com/ecomm/servlet/ ... &langId=-1
I hope this helps. Good luck! Let us know if you have any other questions.