Staphylococcus Aureus help?

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ASAP PLEASE! Procedures for S. Aureus experiment???

Postby saureusproject » Sun Oct 14, 2012 10:30 am

So my experiment is to find which RGP contact lens storing solution is more effective than other brands in prohibiting growth of Staphylococcus Aureus. I'm confused about how to write the procedure. Questions:

How would I introduce S. Aureus into the different solutions? (there are five lens solutions being tested) Would I do it in a test tube or petri dish, and how? I'm really unsure how to culture S. Aureus!
After culturing, I plan on streaking each individual solution onto separate agar plates and incubating them. Or is culturing and incubating the same thing?
Also, is mannitol salt agar the best agar to grow S. Aureus on?

I would greatly appreciate help on my procure ASAP! Thanks!
saureusproject
 
Posts: 2
Joined: Sun Oct 14, 2012 9:43 am
Occupation: Student: 8th grade
Project Question: Topic: To find out which contact lens solution stops growth of S. Aureus most effectively.
Need help developing in-detail procedure.
Project Due Date: Around November 2012 (not confirmed)
Project Status: I am conducting my research

Staphylococcus Aureus help?

Postby saureusproject » Sun Oct 14, 2012 11:48 am

How would you inoculate Staphyloccocus Aureus into a solution? Any efficient methods?
Thanks!
saureusproject
 
Posts: 2
Joined: Sun Oct 14, 2012 9:43 am
Occupation: Student: 8th grade
Project Question: Topic: To find out which contact lens solution stops growth of S. Aureus most effectively.
Need help developing in-detail procedure.
Project Due Date: Around November 2012 (not confirmed)
Project Status: I am conducting my research

Re: Staphylococcus Aureus help?

Postby donnahardy2 » Sun Oct 14, 2012 6:23 pm

Hi,

Welcome to Science Buddies. This is a really great project idea! I think you might be able to adapt one of the antimicrobial agent projects on the Science Buddies website to your project.

http://www.sciencebuddies.org/science-f ... p012.shtml

http://www.sciencebuddies.org/science-f ... #procedure

http://www.sciencebuddies.org/science-f ... #procedure

Does this give an idea of what you can do? Remember since this is a science project, you need to obtain measurable results.

Culturing is transferring bacteria to a suitable growth medium; incubating involves placing the broth or agar cultures in a suitable environment to allow time for growth.

If you are not familiar with microbiological techniques, you will find the following information helpful. This information includes details for using sterile technique to inoculate bacteria into growth media.

http://www.sciencebuddies.org/science-f ... ques.shtml

Please post again if you have more questions.

Donna Hardy
donnahardy2
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Joined: Mon Nov 14, 2005 12:45 pm


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