Death Rays: What Duration of Ultraviolet Exposure Kills Bact

[handcok]

Hi I am doing this experiment that I got in this website. I was wondering why a bunsen burner was necessary for the project. It is on the materials list. I am also wondering if it is completely necessary for the 200ul pipette to automatic. Also what is the bent glass rod and why is it necessary. The last thing I need is where to buy a shortwave uv light and what is a good model? Please Help. Thank You.

[sunmoonstars]

Hi,

I see you are doing this project: http://www.sciencebuddies.org/science-fair-projects/project_ideas/MicroBio_p017.shtml

I can help answer your questions:
I was wondering why a bunsen burner was necessary for the project. It is on the materials list. I am also wondering if it is completely necessary for the 200ul pipette to automatic. Also what is the bent glass rod and why is it necessary. The last thing I need is where to buy a shortwave uv light and what is a good model?

The bent glass rod is for spreading the bacteria on the plates. The bunsen burner is to heat the glass rods so you can bend them (and it sterilizes at the same time). The pipettor does not need to be automatic - any tool you can use to acurately measure will work fine. You can get a UV light (and the protective eyewear) at home-supply stores, or on Amazon (something like this one or similar will work fine - http://www.amazon.com/LEDwholesalers-Ultraviolet-LED-flashlight-7202UV395/dp/B001Q70A0G/ref=sr_1_2?ie=UTF8&qid=1350479802&sr=8-2&keywords=uv+light)

Let me know if you have any other questions.
Tonya

[klhjbh62604]

Hello:

Tonya has provided you with great information.

I wanted to add that if you can find sterile inoculating loops, you can use these for your plating rather than using a bunsen burner and glass rods.

Kati