Ask questions about projects relating to: biology, biochemistry, genomics, microbiology, molecular biology, pharmacology/toxicology, zoology, human behavior, archeology, anthropology, political science, sociology, geology, environmental science, oceanography, seismology, weather, or atmosphere.

Moderators: MelissaB, kgudger, Ray Trent, Moderators

Re: Procedures

Postby VSegarra » Mon Jan 28, 2013 7:51 pm

Hi Daven,
For the low concentration test solution described in 9b you also have to add the antifungal agent to be tested. Your preparation of the low concentratin dilution of the drug depends on your preparation for the high concentration drug mix. I have described the key points of making the solution below:

Preparing the high test concentration:

1 part drug per 1000 parts total of solution (in water)- this is what they mean when they say 1000-fold dilution. Since you only need a bit of the original drug (concentrated) you are measuring volume of drug added in units of teaspoons.

Preparing the low test concentration:

1 part high test concentration solution (from above) per 1000 parts of total solution.
While for the high test concentration you were using the concentrated drug, here you are diluting the dilution prepared above further.

I would not worry so much about your results being correct as much as noting whether they were reproducible.

Hope this helps!
Posts: 56
Joined: Sun Nov 06, 2011 6:48 am
Occupation: Scientist
Project Question: n/a
Project Due Date: n/a
Project Status: Not applicable

Re: Procedures

Postby Megara7 » Sun Feb 03, 2013 2:09 pm

A simple way to dilute the concentration further would be to dilute it using the method Veronica explained above, then use 1 parts low test concentration per 1000 parts of the total solution. This way you are taking the dilute mixture and diluting it even further.

I hope this helps!
“Nobody important? Blimey, that’s amazing. You know that in nine hundred years of time and space and I’ve never met anybody who wasn’t important before.”
— The Eleventh Doctor
Posts: 66
Joined: Fri Nov 11, 2011 3:06 pm
Occupation: Student: 12th grade
Project Question: n/a
Project Due Date: n/a
Project Status: Not applicable

Return to Grades 6-8: Life, Earth, and Social Sciences

Who is online

Users browsing this forum: Yahoo [Bot] and 5 guests