How are you? Did you get your assignment turned in?
As promised, here is some basic information that will be helpful for you to understand your project. You need to read as much as possible and become a subject matter expert on anthocyanins.
This site explains the chemistry of anthocyanins and shows a typical molecular structure. The important points here are to notice that the molecules have three 6-carbon rings with alternating single and double bonds. This is what causes the color of the pigment. Anthocyanins also have a sugar molecule (C6, H11,05) attached.http://www.demochem.de/p26_anth-e.htm
Here is a recent research paper on anthocyanins in wild bananas. Please note the format of the paper and the techniques used the study the anthocyanins. This is an example of a research report on a topic that is similar to your project, and you can use it as a source of ideas. It includes a procedure for extracting the plant pigments and measuring the stability with different pH’s and temperature. http://www.resplantbiol.com/getarticle. ... =5&artid=2
pH is a very important concept to understand for your project because anthocyanins will have different colors at different pH levels. Here is an explanation of pH, or concentration of hydrogen ions, from the Science Buddies website:http://www.sciencebuddies.org/science-f ... cale.shtml
Here is an example of a project, which uses the anthocyanins from purple cabbage to make pH paper:http://www.sciencebuddies.org/science-f ... p041.shtml
You will probably be doing paper chromatography to analyze your samples for this project. Here is an example of a project on paper chromatography. Be sure to read and understand the background section of this project:http://www.sciencebuddies.org/science-f ... background
Unfortunately there is no kit available to purchase for a paper chromatography science project, but here is information for obtaining the supplies you will need. You should check with your teacher and ask if any of the materials are available through your school http://www.sciencebuddies.org/science-f ... rces.shtml
Now, here are some questions for you to think about.
1. What is your research question? This is going to be the key for designing a really good experiment. http://www.sciencebuddies.org/science-f ... tion.shtml
2. What type of flowers (or other colored plants) do you have available to use?
3. Do you have any questions about the background reading material? Can you answer the following?
-what causes the color of anthocyanins?
-what is the purpose of the sugar molecule on the anthocyanin molecule?
-what is the effect of changing pH on the color of anthocyanins?
-what is the best way to extract the anthocyanins from flowers?
-what is Rf in paper chromatography?
-using paper chromatography, how will you compare results from different samples?
-what is your independent and dependent variable?
-why can’t you use electrophoresis to separate anthocyanins?
Please let me know if you have questions. I know that there’s lots of chemistry and new information for you here, but I need to make sure that you understand the basics before you continue. It’s very important to understand the science behind your project because this will help you design a better experiment.
When is your next deadline?