I read your thoughts on the chemical make up of the tangerine lemon and lime seed oil extracts. I had the chemical make up for all. What stood out was tangerine has a chemical component in it called alpha -thujone while the others lack this component. Alpha-thujone is more known in wormwood and after researching it, I found it has antibacterial qualities. All oils used seem to have some antibacterial qualities, but tangerine has this extra one. Since algae and bacteria live in symbiosis, this could be a reason why it works better. Algae gives off o2 and needs co2. Bacteria gives off co2 and needs o2. COZY!!! If there were bacteria in the sample sent to me, perhaps the tangerine effected it more than the lime and lemon!!!! This would make sense
I ran the experiment twice. I couldn't run more because the counting was making me nauseas and caused me a lot of neck pain. I hope they accept this. I wanted to run more but the thought of it made me sick.
My raw data follows. The numbers have already been multiplied by 5 to account for the 1:5 dilution factor
Cells counted must still be x10 to 4th for the cellometer per manufacturer
1:10. 1:100. 1:1000
Con. 1555/1660 1465/1550. 1475/1490.
Ag. 730/770. 1075/1025. 1075/1135
Gr. 1120/1065. 1260/1220. 1280/1300
Lem. 1260/1230. 1299/1450. 1255/1220
Lim. 1225/1270. 1339/1310. 1420/1430
Tang. 1070/1165. 1295/1280. 1440/1450
I worked out the percentages of cell loss based on the averages between the two test runs. I took the average cell count of an algaecide and divided it by the average control cell count for each dilution group.. If I got 47% for Ag at the 1:10 dilution, I said there was a 53% cell loss. Does his sound right?
I have controlled variables....lighting, temp, same lot of Ankistrodesmus introduced to each sample, time for counting, all used same spring water for dilutions.
Independent variables are the different dilutions of the algaecdies.. Used for cellometer counting ...1ml Ankistrodesmus and 4 ml of the algaecides
Dependent variable is the amount of cell death.
Control is 1ml Ankistrodesmus And 4ml spring water to use in cellometer
It is a bit involved. Does it sound Ok?