How long should one incubate E. coli?

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How long should one incubate E. coli?

Postby a380bg » Sun Nov 18, 2012 1:33 pm

I want to test the effects of UV light on the survival of E. coli. I am wondering how long I should incubate the dish with samples from the surface that was exposed to uv light.
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Re: How long should one incubate E. coli?

Postby donnahardy2 » Sun Nov 18, 2012 7:06 pm

Hi,

Welcome to Science Buddies! Are you planning to do this excellent, but challenging project?

http://www.sciencebuddies.org/science-f ... p017.shtml

E. coli should be able to grow with overnight incubation at 37 degrees Centigrade or within two days at ambient temperature. UV light will damage the cells, so it may take a few hours for the bacteria to recover and start growing again, so if there are no colonies after 24 hours, incubate the plates another day or two to verify the results. Be sure and use a positive control that is not exposed to UV light in case you have killed all of the bacteria, and a negative control, if possible to verify your agar plates are sterile.

Please post again if you have any questions.


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Re: How long should one incubate E. coli?

Postby a380bg » Sun Nov 18, 2012 7:30 pm

Thanks for the quick response! I was asking for a friend in my class.
a380bg
 
Posts: 4
Joined: Sun Nov 18, 2012 11:16 am
Occupation: Student:10th Grade
Project Question: The survival of pro-biotic bacteria in the presence of substances common in processed foods.(Are processed foods bad for intestinal flora)
Project Due Date: February
Project Status: I am conducting my research


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