Separate dead yeast from live yeast

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Separate dead yeast from live yeast

Postby 145flute » Wed Nov 28, 2012 6:23 pm

For my experiment, I am going to grow yeast and expose it to Electromagnetic waves to see if the waves have an effect on the yeast's growth. What is a cheap (preferably easy) way to differentiate between the living yeast and the dead yeast?
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Occupation: Student: 10th grade
Project Question: The effect of EMF radiation has on yeast.
Project Due Date: 2/20/12
Project Status: Not applicable

Re: Separate dead yeast from live yeast

Postby donnahardy2 » Sun Dec 02, 2012 9:53 am

Hi,

Methylene blue is the most commonly used vital stain to distinguish living from dead yeast cells. Here is a technical note from a company that sells scientific supplies comparing methylene blue with two other stains, Tryphan blue and methylene blue. Vital stains do not stain viable cells, but will stain,non-viable yeast cells, so you can see the difference when using a microscope.. Note that the information contains data to show that all of the stains give comparable results.

https://www.beckmancoulter.com/wsrporta ... Ta-204.doc

Carolina Biologicals offers the dye and ready-to-use staining solutions.

http://www.carolina.com/

You can find other vital stains by doing an internet search for "vital stains for yeast."

It sounds like you are doing a very interesting science project. Please post again if you need any additional information.

Donna Hardy
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