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For my experiment, I am going to grow yeast and expose it to Electromagnetic waves to see if the waves have an effect on the yeast's growth. What is a cheap (preferably easy) way to differentiate between the living yeast and the dead yeast?
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- Joined: Thu Nov 08, 2012 4:15 pm
- Occupation: Student: 10th grade
- Project Question: The effect of EMF radiation has on yeast.
- Project Due Date: 2/20/12
- Project Status: Not applicable
Methylene blue is the most commonly used vital stain to distinguish living from dead yeast cells. Here is a technical note from a company that sells scientific supplies comparing methylene blue with two other stains, Tryphan blue and methylene blue. Vital stains do not stain viable cells, but will stain,non-viable yeast cells, so you can see the difference when using a microscope.. Note that the information contains data to show that all of the stains give comparable results. https://www.beckmancoulter.com/wsrporta ... Ta-204.doc
Carolina Biologicals offers the dye and ready-to-use staining solutions. http://www.carolina.com/
You can find other vital stains by doing an internet search for "vital stains for yeast."
It sounds like you are doing a very interesting science project. Please post again if you need any additional information.
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