Are you using a project from this website? If so, which one is it?
Also what would help me is to find out from you what your hypothesis is. What will you be testing for exactly, such as: "Based on my research, I hypothesize that the active chemical in plant pesticides, known as ________, will inhibit/ prevent, etc. mammalian cell growth. That was only an example.
Here are some safety guidelines you should adhere to: Also, is this project for the ISEF?http://www.sciencebuddies.org/science-f ... ents.shtml
Cell culturing can be lengthy, especially if this is the first time you are doing it. This is an ambitious goal for a 3 month time period. I would work on breaking the project into a smaller chunk, such as: obtaining the pure chemical from pesticides and purchasing previously established cell lines. You could measure different pesticide concentrations (of the same chemical), to see how it affects growth/ deterioration of cells. This would not be a molecular project since you would be observing cellular components, i.e. do the cells die off, change shape, deteriorate, etc? You will need a compound microscope for this.
Here is more info on cell culture:http://en.wikipedia.org/wiki/Cell_culture
Also, you will need to work in a professional scientific lab, either through a college, university, or laboratory facility because you will be working with hazardous chemicals, biological agents, toxic materials. Extreme safety precautions need to be taken, and you will need a supervisor. I'm not sure if someone at your level would be able to obtain or use mammalian cells, so you should check with your school or science lab.
Here are some other ideas similar to what youre interested in:
This project is very similar to your interests, but less dangerous, costly, and time consuming: http://www.sciencebuddies.org/science-f ... ml#summaryhttp://www.sciencebuddies.org/science-f ... ml#summaryhttp://www.sciencebuddies.org/science-f ... p018.shtml
Let us know if you need further help or have questions about any of this. Happy Holidays!