aparna wrote:I have a doubt regarding the purification of plant extracts before doing HPLC. Is it necessary to remove the chlorophyll ?
One more thing, which solvent system is most suitable for doing TLC for the separation of plant extracts ?
aparna wrote:Thanks a lot for your suggestion.But i thought of doing TLC inorder to check the suitable solvent system so that it will be easier for me to run a Column Chromatography.
aparna wrote:What reaction occurs when a plant methanol extract with dilute HCl is heated with activated charcoal ? i doubt it because before treatment with charcoal, i had a light green colored solution.After that, it turned to dark red colored clear solution.
Please do help.
aparna wrote:Thanks for your suggestions.Well, let me explain my project.My aim is to check the anti inflammatory component present in a particular medicinal plant.For that, i need a good protocol to obtain the plant extract without the presence of chlorophyll. Only if i get a pure sample, i can go for an HPLC if possible.Otherwise i will end up in doing TLC and Column chromatography.To find the exact component, more experiments has to be worked out as per my professor says.My lab has got average requirements to do my work.But i hope i get more exposure to other labs.Now i am trying out different protocols to isolate the alkaloids and also organic components present in that plant.
Hope you understand my work now.I am following the basic protocols seen in google for isolation.
aparna wrote:Thanks again.........Sorry for the trouble.
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