Effect of pH on pectinase

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Effect of pH on pectinase

Postby jm001 » Sun Mar 03, 2013 2:38 pm

I am doing an experiment on the effect of pH on pectinase and I wasn't sure how to change the pH correctly. Do I need to use a buffer or, for example, can I just add hydrochloric acid to the pectinase solution?
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Project Question: Effect of pH on the enzyme pectinase
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Re: Effect of pH on pectinase

Postby Goldenzenith » Sun Mar 10, 2013 10:25 pm

Hi jm001,

I've heard of kids doing this experiment by adding things like HCl, vinegar, and even bleach! If you had any hesitations using HCl, brush them off! I think it's fine to use that, so long as you're careful, of course. :) If you want, there's no harm in trying the buffer and seeing if anything happens; how would the results compare to, say, what you hypothesized? It'd be interesting to find out! Have fun and good luck!
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