My understanding of the rules is that invertebrates do not fall under the ISEF tissue regulations even if they are euthanized. However, use of tricaine methanesulfonate will require, at the very least, the completion of a Risk Assessment Form http://www.societyforscience.org/document.doc?id=18 prior to starting the experiments. I find it difficult however, from my reading of the rules, to assess whether these are experiments that are allowed as "home" experiments. Given that the last thing you want to do is be disqualified by a rule technicality, you should contact your local fair's scientific review committee (SRC) and directly address your questions to them. You can start this process by calling or e-mailing the science fair coordinator, they are likely to be listed on the fair's website. The ISEF website also urges students to: Contact the firstname.lastname@example.org with specific rules questions. I strongly recommend you do this.
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