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Abstract

Many proteins act as catalysts which speed up or regulate a biochemical reaction. The kinetics of an enzyme can be investigated by measuring the reactants and products of the reaction over time. Research the enzymatic reaction of a protein and investigate its kinetics. Measure the production of hydrogen peroxide (H2O2) by catalase, the digestion of gelatin by papain, or any other enzymatic reaction you have access to. Once you have a method for measuring the kinetics of the reaction, conduct a series of experiments to measure the enzyme kinetics under different conditions. For example, you could systematically change the concentration of dissolved salts, or the temperature, or the pH of the solution in which you are measuring the enzyme kinetics. Or you could add an excess of one or more reactants, or an excess of one or more products to see what effect this would have on the enzyme kinetics.

Variations


Last edit date: 2008-11-10 13:47:58

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Project Summary

Difficulty  7  –  9 
Time required Long (a couple of weeks)
Prerequisites Should have basic knowledge of chemistry and chemical reactions.
Material Availability Readily available
Cost Low ($20 - $50)
Safety Use caution when choosing a biochemical reaction to study. For more information about chemical safety, consult the Chemical Safety Guide


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Sponsored by generous grants from Bio-Rad and its Biotechnology Explorer program

Inspiring minds want to know.
Unravel the mysteries of biotech
explorer.bio-rad.com

Career Focus

If you like this project, you might enjoy exploring related careers.

Chemist
Everything in the environment, whether naturally occurring or of human design, is composed of chemicals. Chemists search for and use new knowledge about chemicals to develop new processes or products.
  Biochemist
Growing, aging, digesting—all of these are examples of chemical processes performed by living organisms. Biochemists study how these types of chemical actions happen in cells and tissues, and monitor what effects new substances, like food additives and medicines, have on living organisms.




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