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Expression Cloning *

Difficulty
Time Required Average (6-10 days)
Prerequisites Some laboratory experience is required: knowledge of sterile technique, working with bacterial cultures, and using automatic pipettes are all helpful.
Material Availability In the Summary tab change the Material Availability field to read: This science project requires the purchase of Bio-Rad's pGLO Bacterial Transformation Kit. You will need a teacher's help to order the kit as Bio-Rad only sells directly to schools.
Cost Very High (over $150)
Safety Adult supervision is required in the laboratory facility. Use sterile technique. Read the Microorganisms Safety Guide before starting any experiments. SRC approval may be necessary.
*Note: This is an abbreviated Project Idea, without notes to start your background research, a specific list of materials, or a procedure for how to do the experiment. You can identify abbreviated Project Ideas by the asterisk at the end of the title. If you want a Project Idea with full instructions, please pick one without an asterisk.

Abstract

What is cloning and how is it used in the biotechnology industry? How are plasmids constructed for use in a bacterial expression system? You can investigate bacterial expression with the pGLO Bacterial Transformation Kit from the Bio-Rad Biotechnology Explorer Program to make bacteria express green fluorescent protein (GFP), as shown in Figure 1, below (see the Science Buddies project, Bacterial Transformation Efficiency). With variations to this kit you can test the conditions for pGLO expression under different conditions. You can also combine with other kits to map the pGLO plasmid and purify the expressed pGLO protein using column chromatography. (Bio-Rad, 2006)

Picture of plate with GFP bacteria.
Figure 1. You can investigate bacteria expressing GFP using the pGLO Bacterial Transformation Kit. (Photo courtesy of Bio-Rad Laboratories, Inc.)

Cite This Page

MLA Style

Science Buddies Staff. "Expression Cloning" Science Buddies. Science Buddies, 22 Oct. 2014. Web. 23 Nov. 2014 <http://www.sciencebuddies.org/science-fair-projects/project_ideas/BioChem_p024.shtml>

APA Style

Science Buddies Staff. (2014, October 22). Expression Cloning. Retrieved November 23, 2014 from http://www.sciencebuddies.org/science-fair-projects/project_ideas/BioChem_p024.shtml

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Last edit date: 2014-10-22

Bibliography

Bio-Rad, 2006. "Biotechnology Explorer Program," Bio-Rad Laboratories [accessed January 5, 2009] http://explorer.bio-rad.com

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Materials and Equipment

Note: Bio-Rad Kits are sold directly to schools. To purchase, please have your school contact Bio-Rad at 800-424-6723 to verify account information and to place the order for you. Existing accounts will have orders processed within a day, and establishing an account will take approximately 48 hours.

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