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Project Summary
| Difficulty | 8 – 10 |
| Time required | Average (about one week) to Long (a couple of weeks) |
| Prerequisites | Some laboratory experience is required: knowledge of sterile technique, working with bacterial cultures, and using automatic pipettes are all helpful. |
| Material Availability | Kit available for purchase online from www.bio-rad.com, catalog #166-0003EDU |
| Cost | Very High (over $150) |
| Safety | Adult supervision is required in the laboratory facility. Use sterile technique. Read the Microorganisms Safety Guide before starting any experiments. SRC approval may be necessary. |
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Sponsor
| Sponsored by generous grants from Bio-Rad and its Biotechnology Explorer program |
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| Inspiring minds want to know. Unravel the mysteries of biotech |
| explorer.bio-rad.com |
| * Note: This is an abbreviated project idea, without notes to start your background research or a procedure for how to do the experiment. You can identify abbreviated project ideas by the asterisk at the end of the title. If you want a project idea with full instructions, please pick one without an asterisk. |
Abstract
What is cloning and how is it used in the biotechnology industry? How are plasmids constructed for use in a bacterial expression system? You can investigate bacterial expression with the pGLO Bacterial Transformation Kit (Catalog #166-0003EDU) from the Bio-Rad Biotechnology Explorer Program (see the Science Buddies project, Bacterial Transformation Efficiency). With variations to this kit you can test the conditions for pGLO expression under different conditions. You can also combine with other kits to map the pGLO plasmid and purify the expressed pGLO protein using column chromatography. (Bio-Rad, 2006)Bibliography
Bio-Rad, 2006. "Biotechnology Explorer Program," Bio-Rad Laboratories [accessed January 5, 2009] http://explorer.bio-rad.com
Materials and Equipment
Note: Bio-Rad Kits are sold directly to schools. To purchase, please have your school contact Bio-Rad at 800-424-6723 to verify account information and to place the order for you. Existing accounts will have orders processed within a day, and establishing an account will take approximately 48 hours.Variations
- For more science project ideas in this area of science, see Biotechnology Project Ideas.
Last edit date: 2009-01-05 12:00:00
Career Focus
If you like this project, you might want to think about career opportunities in
Biotechnology.
Growing, aging, digesting—all of these are examples of chemical processes performed by living organisms. Biochemists study how these types of chemical actions happen in cells and tissues, and monitor what effects new substances, like food additives and medicines, have on living organisms. Learn more about this career: Biochemist.
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