Purification Strategies *
| Difficulty | |
| Time Required | Average (6-10 days) |
| Material Availability | Most items are readily available; any specialty items needed can be ordered from online vendors. |
| Cost | Average ($50 - $100) |
| Safety | If bacteria or cell lines are used as the source of material for purification, SRC approval may be necessary. Adult supervision is recommended. |
*Note: This is an abbreviated Project Idea, without notes to start your background research, a specific list of materials, or a procedure for how to do the experiment. You can identify abbreviated Project Ideas by the asterisk at the end of the title. If you want a Project Idea with full instructions, please pick one without an asterisk.
Abstract
In order to study individual biochemical compounds like proteins, DNA, or RNA, biochemists need to know how to purify these components from a complex mixture. This is especially important for biotechnology and pharmaceutical industries, which sell purified biochemicals as reagents or drugs to consumers. Do an experiment to purify DNA, RNA, or protein from a complex mixture (for purifying DNA, see the Science Buddies project Extracting Onion DNA). The source of the material can be a cell line, bacterial culture, plant extract, or yeast culture. Which purification strategies work best for your compound of interest? Can you use enzymes like protease, DNAse, or RNAse to test the product of your purification to see if it worked? Are there ways of altering the protocol to make it work better and increase the yield? For example, you could try changing detergent concentrations, salinity, or pH, or adding enzymes.Share your story with Science Buddies!
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Last edit date: 2013-01-10
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