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Microbiology Techniques & Troubleshooting

Science Buddies is continuing to develop tools and techniques to aid you with your Microbiology science projects. Please revisit this page as our resources grow.

Storing Bacterial Strains How to store a bacterial strain for later use
Microorganisms Safety Guide


Other Microbiology Resources

The following Microbiology resources were developed by the Department of Biology at the University of Missouri in St. Louis and can be found at: http://www.umsl.edu/~microbes/techniques.html
   
Culture Problems Descriptions of some common problems and solutions to those problems
Sterile Technique Methods for sterile transfer of cultures in broth or on plates
Optimum Temperatures Optimum temperatures for growth of common lab strains
Sterilizing Methods for sterilizing media and glassware in the classroom
Streaking plates Method for streaking plates to obtain isolated colonies
Nutrient Agar Instructions for preparing nutrient broth, plates, and slants
Tips for plates Tips for pouring and storing plates
Incubator A low-cost incubator for the classroom
Homemade Media An inexpensive alternative to commercial media
Testing Autoclaves Commercial spore solutions test sterilizers
Tyndallization An old-fashioned method for sterilizing broth
Simple Staining of Microbes Instructions for staining bacteria

Source

University of Missouri, St. Louis Department of Biology. (n.d.). Techniques and Troubleshooting. Science in the Real World: Microbes in Action Program. Retrieved April 23, 2008 from http://www.umsl.edu/~microbes/techniques.html

 

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