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Others Like “Protein Fingerprinting”

Science Fair Project Idea
What is cloning and how is it used in the biotechnology industry? How are plasmids constructed for use in a bacterial expression system? You can investigate bacterial expression with the from the Bio-Rad Biotechnology Explorer Program (see the Science Buddies project, ). With variations to this kit you can test the conditions for pGLO expression under different conditions. You can also combine with other kits to map the pGLO plasmid and purify the expressed pGLO protein using column… Read more
BioChem_p024
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Time Required Average (6-10 days)
Prerequisites Some laboratory experience is required: knowledge of sterile technique, working with bacterial cultures, and using automatic pipettes are all helpful.
Material Availability In the Summary tab change the Material Availability field to read: This science project requires the purchase of Bio-Rad's [# Link Name="BioChem_p035.6" Value="HtmlAnchor" #]. You will need a teacher's help to order the kit as Bio-Rad only sells directly to schools.
Cost Very High (over $150)
Safety Adult supervision is required in the laboratory facility. Use sterile technique. Read the [# ProjectGuide Name="Advanced.MicroorganismsSafetyGuide" Value="HtmlAnchor" HtmlShortTitle="true" #] before starting any experiments. [# ProjectGuide Name="Advanced.ScientificReviewCommitteeSRC" Value="HtmlAnchor" HtmlText="SRC approval" #] may be necessary.
Science Fair Project Idea
Are you interested in the technical and ethical aspects of genetic screening, forensic science, and DNA fingerprinting? Scientists use different forms of a gene, called alleles, to differentiate between two fingerprints. One such sequence used in human DNA fingerprinting is the Alu repeat (a 300 base pair repetitive sequence of DNA) on chromosome 16. During evolutionary time, up to 1 million copies of the Alu repeat have become randomly inserted throughout the human genome. Within a specific… Read more
BioChem_p022
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Time Required Very Long (1+ months)
Prerequisites Access to a research laboratory with PCR equipment is required for this project.
Material Availability [# Link Name="BioChem_p022.5" Value="HtmlAnchor" #] available for purchase online from Bio-Rad. A teacher's help is required as Bio-Rad only sells directly to schools.
Science Fair Project Idea
Have your favorite foods been genetically modified? Genetically modified (GM) foods do not require labeling in the USA, and foods with less than 5% content from genetically modified organisms (GMOs) can be labeled "GMO free." In Europe and Asia, GM foods require labeling only if they contain more than 1% GM content. You can investigate whether your favorite food products, like corn tortilla chips, are made from genetically modified organisms using the from the Bio-Rad Biotechnology Explorer… Read more
BioChem_p025
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Time Required Average (6-10 days)
Prerequisites Access to a research laboratory with PCR equipment is required for this project.
Material Availability Requires purchase of Bio-Rad's [# Link Name="BioChem_p025.1" Value="HtmlAnchor" #]. A teacher's help is needed to order as Bio-Rad sells directly to schools.
Cost Very High (over $150)
Safety Adult supervision is required in the laboratory facility.
Science Fair Project Idea
thumbnail Do you like reading detective stories or watching crime shows on TV? This advanced project idea in the area of forensic science might be right for you. Read more
BioChem_p009
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Time Required Average (6-10 days)
Prerequisites High School Biology
Material Availability This project requires you to order specialty items and have access to some laboratory equipment, see the Materials & Equipment section for more details.
Cost High ($100 - $150)
Safety No issues
Science Fair Project Idea
Many proteins act as catalysts which speed up or regulate a biochemical reaction. The kinetics of an enzyme can be investigated by measuring the reactants and products of the reaction over time. Research the enzymatic reaction of a protein and investigate its kinetics. Measure the production of hydrogen peroxide (H2O2) by catalase, the digestion of gelatin by papain, or any other enzymatic reaction you have access to. Bio-Rad's provides a great measurement platform for exploring… Read more
BioChem_p019
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Time Required Long (2-4 weeks)
Prerequisites Should have basic knowledge of chemistry and chemical reactions.
Material Availability Readily available
Cost Low ($20 - $50)
Safety Use caution when choosing a biochemical reaction to study. For more information about chemical safety, consult the [# ProjectGuide Name="Advanced.ChemistrySafetyGuide" Value="HtmlAnchor" #] Guide.
Science Fair Project Idea
thumbnail Have you ever had to sort a jumble of objects into piles, based on their type? Maybe laundry, or a big load of dishes, or while organizing the garage or a closet? Scientists have to do something similar when they want to study or isolate just a single type of protein. For example, a botanist might discover an exotic plant that is poisonous when eaten, but that also has great antibiotic properties. To help develop a new antibiotic for human use, he or she would have to separate the different… Read more
BioChem_p035
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Time Required Average (6-10 days)
Prerequisites Familiarity with how to use a micropipette is needed.
Material Availability This science fair project requires specialty items, including the Bio-Rad [# Link Name="BioChem_p035.5" Value="HtmlAnchor" #] and a sample of green fluorescent protein. See the Materials and Equipment list for details. It also requires a number of common molecular biology items, such as pipettes, micropipettes and tips, and a UV light—likely obtainable from your school laboratory.
Cost Very High (over $150)
Safety Adult supervision is recommended. Follow all safety instructions that come with the Bio-Rad kit(s).
Science Fair Project Idea
thumbnail Imagine that you want to create your own brand of colored candies. You know they would be a success if only you could figure out how to make a certain color, such as purple. How can you do this when government regulators have approved just a few food dyes? First, you will need to find out how your competitors make all of their colors from these few dyes. Do different candy brands use the same or different dyes? This can be figured out with gel electrophoresis, a technique commonly used in… Read more
BioChem_p039
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Time Required Short (2-5 days)
Prerequisites None
Material Availability This project requires a [# Link Name="BioChem_p039.1" Value="HtmlAnchor" #]. See the Materials and Equipment list for details.
Cost High ($100 - $150)
Safety Use caution when heating the agarose. Never connect more than five 9-volt (V) batteries together for this project.
Science Fair Project Idea
thumbnail When biologists want to separate different pieces of DNA, RNA, or proteins they use a technique called gel electrophoresis. In this science project you'll build a gel electrophoresis chamber and use it to discover how many components are in different colors of food coloring dye. Read more
BioChem_p028
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Time Required Short (2-5 days)
Prerequisites None
Material Availability Readily available
Cost Average ($50 - $100)
Safety No issues
Science Fair Project Idea
thumbnail Are biofuels the wave of the future? People often talk about these plant-derived fuels as a way to someday cut down on our dependency on non-renewable carbon-based fuels, like gasoline. Ethanol (a type of alcohol) is a common biofuel used today. In the United States, ethanol is a common biofuel additive to normal gasoline. In fact, some states mandate that when you fill up your gas tank, 10 percent of the total fuel volume be made of ethanol. Brazil, the world's largest user of ethanol-based… Read more
BioChem_p038
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Time Required Long (2-4 weeks)
Prerequisites This science project requires access to a biology or chemistry laboratory with, at minimum, pipettes and a balance.
Material Availability This project requires the Bio-Rad [# Link Name="BioChem_p019.1" Value="HtmlAnchor" #]. You will need a teacher's help to order it as Bio-Rad only sells directly to schools.
Cost Very High (over $150)
Safety Adult supervision is required in the laboratory facility.
Science Fair Project Idea
In order to study individual biochemical compounds like proteins, DNA, or RNA, biochemists need to know how to purify these components from a complex mixture. This is especially important for biotechnology and pharmaceutical industries, which sell purified biochemicals as reagents or drugs to consumers. Do an experiment to purify DNA, RNA, or protein from a complex mixture (for purifying DNA, see the Science Buddies project ). The source of the material can be a cell line, bacterial culture,… Read more
BioChem_p026
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Time Required Average (6-10 days)
Material Availability Most items are readily available; any specialty items needed can be ordered from [# ProjectGuide Name="Advanced.ApprovedSuppliers" Value="HtmlAnchor" HtmlText="online vendors" #].
Cost Average ($50 - $100)
Safety If bacteria or cell lines are used as the source of material for purification, [# ProjectGuide Name="Advanced.ScientificReviewCommitteeSRC" Value="HtmlAnchor" HtmlText="SRC approval" #] may be necessary. Adult supervision is recommended.
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