Home Project Ideas Project Guide Ask An Expert Blog Careers Teachers Parents Students

Others Like “Protein Fingerprinting”

Project Idea
What is cloning and how is it used in the biotechnology industry? How are plasmids constructed for use in a bacterial expression system? You can investigate bacterial expression with the pGLO Bacterial Transformation Kit from the Bio-Rad Biotechnology Explorer Program (see the Science Buddies project, Bacterial Transformation Efficiency). With variations to this kit you can test the conditions for pGLO expression under different conditions. You can also combine with other kits to… Read more
BioChem_p024
+ More Details
- Less Details
Time Required Average (6-10 days)
Prerequisites Some laboratory experience is required: knowledge of sterile technique, working with bacterial cultures, and using automatic pipettes are all helpful.
Material Availability In the Summary tab change the Material Availability field to read: This science project requires the purchase of Bio-Rad's pGLO Bacterial Transformation Kit. You will need a teacher's help to order the kit as Bio-Rad only sells directly to schools.
Cost Very High (over $150)
Safety Adult supervision is required in the laboratory facility. Use sterile technique. Read the Microorganisms Safety Guide before starting any experiments. SRC approval may be necessary.
Project Idea
thumbnail Do you like reading detective stories or watching crime shows on TV? This advanced project idea in the area of forensic science might be right for you. Read more
BioChem_p009
+ More Details
- Less Details
Time Required Average (6-10 days)
Prerequisites High School Biology
Material Availability This project requires you to order specialty items and have access to some laboratory equipment, see the Materials & Equipment section for more details.
Cost High ($100 - $150)
Safety No issues
Project Idea
thumbnail Have you ever had to sort a jumble of objects into piles, based on their type? Maybe laundry, or a big load of dishes, or while organizing the garage or a closet? Scientists have to do something similar when they want to study or isolate just a single type of protein. For example, a botanist might discover an exotic plant that is poisonous when eaten, but that also has great antibiotic properties. To help develop a new antibiotic for human use, he or she would have to separate the different… Read more
BioChem_p035
+ More Details
- Less Details
Time Required Average (6-10 days)
Prerequisites Familiarity with how to use a micropipette is needed.
Material Availability This science fair project requires specialty items, including the Bio-Rad Size Exclusion Chromatography Kit and a sample of green fluorescent protein. See the Materials and Equipment list for details. It also requires a number of common molecular biology items, such as pipettes, micropipettes and tips, and a UV light—likely obtainable from your school laboratory.
Cost Very High (over $150)
Safety Adult supervision is recommended. Follow all safety instructions that come with the Bio-Rad kit(s).
Project Idea
Are you interested in the technical and ethical aspects of genetic screening, forensic science, and DNA fingerprinting? Scientists use different forms of a gene, called alleles, to differentiate between two fingerprints. One such sequence used in human DNA fingerprinting is the Alu repeat (a 300 base pair repetitive sequence of DNA) on chromosome 16. During evolutionary time, up to 1 million copies of the Alu repeat have become randomly inserted throughout the human genome. Within a specific… Read more
BioChem_p022
+ More Details
- Less Details
Time Required Very Long (1+ months)
Prerequisites Access to a research laboratory with PCR equipment is required for this project.
Material Availability PV92 PCR Informatics Kit available for purchase online from Bio-Rad. A teacher's help is required as Bio-Rad only sells directly to schools.
Project Idea
Is it possible to manipulate bacteria to become protein production factories? Can diabetics control blood glucose with insulin produced by bacteria? How cool would it be to take advantage of these microorganism's sophisticated makeup, short doubling times and cheap growth media to mass produce medically and commercially useful proteins? All of these are possible with a few simple genetic manipulations. By the end of this project you would know the basic foundation on which many… Read more
BioChem_p013
+ More Details
- Less Details
Time Required Average (6-10 days)
Prerequisites Some laboratory experience required: knowledge of sterile technique, working with bacterial cultures, and using automatic pipets all helpful.
Material Availability Specialty items
Cost Average ($50 - $100)
Safety Requires adult supervision in a laboratory facility. For ISEF-affiliated fairs, this project will require [# ProjectGuide Name="Advanced.ScientificReviewCommitteeSRC" Value="HtmlAnchor" HtmlText="SRC" #] approval.
Project Idea
thumbnail Many people you know probably have an opinion about the kind of milk they like to drink—some like it thin and refreshing, others like it thick and rich. Milk can be bought with different fat concentrations, but other than that, it's all the same. Or is it? This science fair project raises a few interesting questions about the other contents in milk. Do all milk products have the same protein concentrations? Do cows produce different types of milk during different stages of lactation?… Read more
BioChem_p034
+ More Details
- Less Details
Time Required Average (6-10 days)
Prerequisites Prior to starting this science fair project, you should either know how to use a spectrophotometer, or have access to someone who can show you how to use it.
Material Availability This science fair project requires access to laboratory equipment and supplies, including a spectrophotometer; see the Materials and Equipment section for more details. Access to a dairy is also needed for the main project, but the Variations section, below, lists options that are not dependent on dairy access.
Cost Very High (over $150)
Safety Adult supervision is recommended when using laboratory equipment.
Project Idea
thumbnail In the first decade of the 21st century, scientists found ways to make one adult cell type turn into a completely different cell type. This has huge implications for the medical field, including being able to take some cells that a person could spare, such as skin cells or blood cells, and turn them into another cell type that might be much more important for that person to have, such as cells to make a new kidney. How are scientists able to accomplish this amazing feat of "reprogramming" the… Read more
BioMed_p009
+ More Details
- Less Details
Time Required Short (2-5 days)
Prerequisites Basic understanding of how genes are turned into proteins.
Material Availability Readily available
Cost Very Low (under $20)
Safety No issues
Project Idea
thumbnail Find out the real explanation for why your parents are so weird! Here is a project that lets you explore "the net" to find out why your "DNA blueprint" is so important to health and disease. In this project you will use methods that bioinformatics and biotech scientists perform on a daily basis to decipher the human genome in their efforts to diagnose and treat genetic diseases. Read more
Genom_p008
+ More Details
- Less Details
Time Required Average (6-10 days)
Prerequisites A good knowledge of basic concepts in genetics and good computer database searching skills
Material Availability Readily available
Cost Very Low (under $20)
Safety No issues
Project Idea
thumbnail Scientists recently found that some small drugs can stop infection by the deadly Ebola virus in its tracks. Lab researchers found that these drugs bind to a protein that the Ebola virus uses to enter our cells, and this is how infection is prevented. However, this also means that the bound protein no longer functions in our cells. How might these drugs accidentally disrupt important biological processes in our bodies? What other proteins might these drugs bind to? In this science… Read more
BioMed_p011
+ More Details
- Less Details
Time Required Short (2-5 days)
Prerequisites Basic understanding of what viruses, drugs, genes, and proteins are.
Material Availability Readily available
Cost Very Low (under $20)
Safety No issues
Project Idea
How a biological system functions is a consequence of the 3-D structures of biological macromolecules like proteins and protein complexes. Proteins can be categorized into different protein families based upon sequence, structure, and function. Typically, proteins in the same family have similar biochemical functions. You can investigate the structure of a protein by using protein databases (Entrez Protein, SwissProt, PDB) and 3-D computational models. You can compare the structures of… Read more
BioChem_p021
+ More Details
- Less Details
Time Required Very Long (1+ months)
Prerequisites Advanced biology course, or willingness to read up on such topics extensively.
Material Availability A computer with Internet access is necessary for this project.
Cost Very Low (under $20)
Safety No issues
1 2 >
Support for Science Buddies provided by:
Search Refinements
Areas of Science
Behavioral & Social Science
Earth & Environmental Science
Engineering
Life Science
Math & Computer Science
Physical Science
Difficulty
 
Cost
Time
Material Availability