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Others Like “Sizing It Up! How Scientists Separate Proteins”

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Science Fair Project Idea
What is cloning and how is it used in the biotechnology industry? How are plasmids constructed for use in a bacterial expression system? You can investigate bacterial expression with the from the Bio-Rad Biotechnology Explorer Program to make bacteria express green fluorescent protein (GFP), as shown in Figure 1, below (see the Science Buddies project, ). With variations to this kit you can test the conditions for pGLO expression under different conditions. You can also combine with… Read more
BioChem_p024
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Time Required Average (6-10 days)
Prerequisites Some laboratory experience is required: knowledge of sterile technique, working with bacterial cultures, and using automatic pipettes are all helpful.
Material Availability This science project requires the purchase of Bio-Rad's [# Link Name="BioChem_p035.6" Value="HtmlAnchor" #]. Details about how to order products from Bio-Rad can be found [# Page Name="BioRad.Purchasing.Kits" Value="HtmlAnchor" HtmlText="here" #].
Cost Very High (over $150)
Safety Adult supervision is required in the laboratory facility. Use sterile technique. Read the [# ProjectGuide Name="Advanced.MicroorganismsSafetyGuide" Value="HtmlAnchor" HtmlShortTitle="true" #] before starting any experiments. [# ProjectGuide Name="Advanced.ScientificReviewCommitteeSRC" Value="HtmlAnchor" HtmlText="SRC approval" #] may be necessary.
Science Fair Project Idea
Is it possible to manipulate bacteria to become protein production factories? Can diabetics control blood glucose with insulin produced by bacteria? How cool would it be to take advantage of these microorganism's sophisticated makeup, short doubling times and cheap growth media to mass produce medically and commercially useful proteins? All of these are possible with a few simple genetic manipulations. By the end of this project you would know the basic foundation on which many… Read more
BioChem_p013
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Time Required Average (6-10 days)
Prerequisites Some laboratory experience required: knowledge of sterile technique, working with bacterial cultures, and using automatic pipets all helpful.
Material Availability Specialty items
Cost Average ($50 - $100)
Safety Requires adult supervision in a laboratory facility. For ISEF-affiliated fairs, this project will require [# ProjectGuide Name="Advanced.ScientificReviewCommitteeSRC" Value="HtmlAnchor" HtmlText="SRC" #] approval.
Science Fair Project Idea
Many people you know probably have an opinion about the kind of milk they like to drink—some like it thin and refreshing, others like it thick and rich. Milk can be bought with different fat concentrations, but other than that, it's all the same. Or is it? This science fair project raises a few interesting questions about the other contents in milk. Do all milk products have the same protein concentrations? Do cows produce different types of milk during different stages of lactation?… Read more
BioChem_p034
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Time Required Average (6-10 days)
Prerequisites Prior to starting this science fair project, you should either know how to use a spectrophotometer, or have access to someone who can show you how to use it.
Material Availability This science fair project requires access to laboratory equipment and supplies, including a spectrophotometer; see the Materials and Equipment section for more details. Access to a dairy is also needed for the main project, but the Variations section, below, lists options that are not dependent on dairy access.
Cost Very High (over $150)
Safety Adult supervision is recommended when using laboratory equipment.
Science Fair Project Idea
What color is grape soda? If you pour it into a clear glass you can easily see it is purple, but that is usually not its natural color. Manufacturers add red and blue dye to the soda. The dyes mix together and you get purple soda. What if you wanted to un-mix the dyes, could you? Yes! In a chemistry laboratory, using a technique called column chromatography, you could separate the two dyes again. But what about at home, can you use low-tech supplies to do the same thing? In this science… Read more
BioChem_p045
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Time Required Average (6-10 days)
Prerequisites None
Material Availability Syringes (without needles) and Space Sand need to be specially ordered. See the Materials and Equipment list for details.
Cost Low ($20 - $50)
Safety Use caution and follow all safety warnings when handling and using the 70% isopropyl alcohol. The alcohol is highly flammable. Do not swallow, and avoid any contact with eyes.
Science Fair Project Idea
Quick, what is your favorite color of M&Ms® candy? Do you want to know what dyes were used to make that color? Check out this science project to find out how you can do some scientific detective work to find out for yourself. Read more
FoodSci_p006
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Time Required Short (2-5 days)
Prerequisites None
Material Availability A kit is available from the [# Link Name="FoodSci_p006.7" Value="HtmlAnchor" #].
Cost Low ($20 - $50)
Safety No issues
Science Fair Project Idea
DNA is the "instruction manual" for the successful growth of a living thing, from a single cell to a mature adult. When the DNA of an organism is somehow damaged, it can have an impact on the organism's development over time. In this plant science fair project, you will track how irradiation (exposure to radiation) of radish seeds affects germination (sprouting of a seedling from a seed). Read more
PlantBio_p039
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Time Required Long (2-4 weeks)
Prerequisites None
Material Availability You will need a specialty item, Irradiated "Rapid Radish" seeds, for this science fair project. See the Materials and Equipment list for more details.
Cost Average ($50 - $100)
Safety No issues
Science Fair Project Idea
Native plants are important for both ecological and medicinal reasons. However, most native plants are not very well studied. One way to know more about these plants is to understand which other species of plants they are most closely related to. In this science project you will sequence part of the chloroplast genome from a native plant and use this information to determine its evolutionary relationship to other species of plants. If the plant you chose has not been sequenced before, you can… Read more
Genom_p012
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Time Required Long (2-4 weeks)
Prerequisites High school biology class; familiarity with using the PCR machine and gel electrophoresis equipment, and/or using the equipment with a mentor.
Material Availability You will need specialized pieces of equipment, including a PCR machine (thermocycler) and a gel electrophoresis apparatus. If your school does not have this equipment you might want to seek out a mentor at a junior college, university, or biotechnology company. If you have the resources you can do the DNA sequencing yourself, otherwise you can send the samples to Dr. Baysdorfer at California State University, East Bay, see Experimental Procedure for more details.
Cost High ($100 - $150)
Safety This project uses ethidium bromide, which is a carcinogen. Always wear gloves and safety goggles when handling. Dispose of the ethidium bromide according to your school or institute's hazardous waste protocols. If you have concerns, ask your teacher or mentor for advice.
Science Fair Project Idea
Have you ever looked at sunlight through a prism? If so, you know that the prism can separate the sunlight into many different colors of light — a rainbow. Like sunlight, chemical mixtures can also be broken into their component parts. One way of doing this is a simple technique called paper chromatography. What do you think you will see if you use paper chromatography to look at the components of black ink? Is black ink just black? Find out for yourself! Read more
Chem_p008
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Time Required Short (2-5 days)
Prerequisites None
Material Availability A kit is available from the [# Link Name="FoodSci_p006.7" Value="HtmlAnchor" #].
Cost Low ($20 - $50)
Safety No Issues
Science Fair Project Idea
Have your favorite foods been genetically modified? Genetically modified (GM) foods do not require labeling in the USA, and foods with less than 5% content from genetically modified organisms (GMOs) can be labeled "GMO free." In Europe and Asia, GM foods require labeling only if they contain more than 1% GM content. You can investigate whether your favorite food products, like corn tortilla chips, are made from genetically modified organisms using the from the Bio-Rad Biotechnology Explorer… Read more
BioChem_p025
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Time Required Average (6-10 days)
Prerequisites Access to a research laboratory with PCR equipment is required for this project.
Material Availability Requires purchase of Bio-Rad's [# Link Name="BioChem_p025.1" Value="HtmlAnchor" #]. Information about how to order from Bio-Rad can be found [# Page Name="BioRad.Purchasing.Kits" Value="HtmlAnchor" HtmlText="here" #].
Cost Very High (over $150)
Safety Adult supervision is required in the laboratory facility.
Science Fair Project Idea
Are you interested in the technical and ethical aspects of genetic screening, forensic science, and DNA fingerprinting? Scientists use different forms of a gene, called alleles, to differentiate between two fingerprints. One such sequence used in human DNA fingerprinting is the Alu repeat (a 300 base pair repetitive sequence of DNA) on chromosome 16. During evolutionary time, up to 1 million copies of the Alu repeat have become randomly inserted throughout the human genome. Within a specific… Read more
BioChem_p022
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Time Required Very Long (1+ months)
Prerequisites Access to a research laboratory with PCR equipment is required for this project.
Material Availability [# Link Name="BioChem_p022.5" Value="HtmlAnchor" #] available for purchase online from Bio-Rad. See [# Page Name="BioRad.Purchasing.Kits" Value="HtmlAnchor" HtmlText="this page" #] for details on how to purchase kits from Bio-Rad.
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