kpeters12
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Yeast Busters dilution concentration verification

Postby kpeters12 » Mon Nov 06, 2017 2:26 am

Hi
We are trying to verify the low concentration for this experiment. The calculations for the high concentration make sense but is it correct that the low concentration for antifungal medications of 1% would be 10,000- fold dilution which is 5ug/mL concentration of antifungal agent? Thanks!

SciB
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Re: Yeast Busters dilution concentration verification

Postby SciB » Wed Nov 08, 2017 6:48 pm

The calculations are a little tricky but as I read them: https://www.sciencebuddies.org/science- ... #procedure

The LOW concentration of antifungal medication is prepared by diluting 1/8 tsp of the HIGH, 1000-fold solution (10 ug/ml), into 1/4 cup of water (12 tsp or 96 eighth tsp) which is a 100-fold dilution. So, a 100-fold dilution of a 1000-fold dilution is a 100,000-fold dilution if my reasoning is correct.

Other experts please CHECK!!

The high concentration (given that the antifungal chemical is at 1%) is 10 ug/ml, so the low concentration would be 1/100 of that or 0.10 ug/ml.

Does that sound correct to you?

Sybee

kpeters12
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Joined: Mon Nov 06, 2017 2:16 am
Occupation: Other Adult

Re: Yeast Busters dilution concentration verification

Postby kpeters12 » Thu Nov 09, 2017 7:12 am

Hi Sybee
Thanks so much for your response! Your reasoning and calculations make sense to us now that we see the logic you used. Thanks very much for the help! :D


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