Posted: Wed Oct 17, 2007 4:21 pm
I've done some searching and found http://www.usc.edu/CSSF/History/2004/Projects/J0404.pdf and http://www.geocities.com/CapeCanaveral/ ... -B-11.html which appear to be similar extraction proceedures using Isopropyl alchohol. This means that the chemical difference and any solubility differences between alchohols isn't important for this separation step.
In looking back through this thread, you, the researcher, have not stated what your hypothesis is. Several responses by experts have hinted at some potential areas that might be interesting. Without a stated hypothesis, we are not going to be much help in identifying controls and variable types. What you have so far is a demonstration "Can I extract Onion DNA using ... process?". Proving that what you extracted is DNA is a much more difficult problem, but even that does not appear to be the kind of a science project that your teacher wants you to do because it is just a demonstration.
You need to go back and try to understanding the scientific method https://www.sciencebuddies.org/mentorin ... thod.shtml and see how you might form a hypothesis that might use this or a similar extraction process to answer a scientific question that will satisfy your teacher's criteria. Doing demonstrations is usually much easier than applying a scientific method to answering a unique question. Repeating somebody elses demonstration is definitely easier yet. It is not that you aren't learning things, but you aren't "doing your own science" in a way that will stand on its own.
In looking back through this thread, you, the researcher, have not stated what your hypothesis is. Several responses by experts have hinted at some potential areas that might be interesting. Without a stated hypothesis, we are not going to be much help in identifying controls and variable types. What you have so far is a demonstration "Can I extract Onion DNA using ... process?". Proving that what you extracted is DNA is a much more difficult problem, but even that does not appear to be the kind of a science project that your teacher wants you to do because it is just a demonstration.
You need to go back and try to understanding the scientific method https://www.sciencebuddies.org/mentorin ... thod.shtml and see how you might form a hypothesis that might use this or a similar extraction process to answer a scientific question that will satisfy your teacher's criteria. Doing demonstrations is usually much easier than applying a scientific method to answering a unique question. Repeating somebody elses demonstration is definitely easier yet. It is not that you aren't learning things, but you aren't "doing your own science" in a way that will stand on its own.