arnlan
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Re: Bacteria Transformation Efficiency

Postby arnlan » Sun Nov 16, 2014 3:18 pm

Thanks!

In DaY one of the procedures it says rehydrate bacteria, what does that necessarily mean?

To clear something up, the youtube video is not exactly what i will be doing correct?
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-Arnlan

arnlan
Posts: 80
Joined: Sun Nov 24, 2013 12:05 pm
Occupation: Student: 8th grade

Re: Bacteria Transformation Efficiency

Postby arnlan » Sun Nov 16, 2014 3:24 pm

I have also changed up my purpose, is this better than the previous one? :

The main purpose of this project is to synthesize proteins in a cheaper way, by doing it in the most efficient and cost-effective way, so it is important to know how the amount of plasmid effects transformation efficiency. Bacteria are transformed for numerous different reasons. Some of these reasons may include expression of medically useful recombinant proteins such as insulin for treating a disease or vaccines for prevention of disease. Other reasons could be expression of proteins that confer on bacteria the ability to survive in particular environments such as to "clean up" contaminated environments in bioremediation.
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arnlan
Posts: 80
Joined: Sun Nov 24, 2013 12:05 pm
Occupation: Student: 8th grade

Re: Bacteria Transformation Efficiency

Postby arnlan » Sun Nov 16, 2014 5:29 pm

So will my project look like this http://www.phschool.com/science/biology ... ysis1.html?

The only difference of my project and that is i will have 1 more plate for the LB:AMP:ARA. And also in my project i will be using different amounts of plasmids. So another difference is that i will have ampicillin in all my plates. AM i correct?
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-Arnlan

connief
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Re: Bacteria Transformation Efficiency

Postby connief » Sun Nov 16, 2014 5:54 pm

Yes, all that sounds good. As for "rehydrating bacteria", that depends on where you're getting your bacterial stock. What I'm assuming they're meaning is to resuspend the bacteria in a bit of nutrient broth and then streaking it out on a plate. Are you going to be taking your bacteria from colonies already growing on a plate or in liquid culture, or are you taking them from a frozen stock?

arnlan
Posts: 80
Joined: Sun Nov 24, 2013 12:05 pm
Occupation: Student: 8th grade

Re: Bacteria Transformation Efficiency

Postby arnlan » Sun Nov 16, 2014 7:42 pm

connief wrote:Yes, all that sounds good. As for "rehydrating bacteria", that depends on where you're getting your bacterial stock. What I'm assuming they're meaning is to resuspend the bacteria in a bit of nutrient broth and then streaking it out on a plate. Are you going to be taking your bacteria from colonies already growing on a plate or in liquid culture, or are you taking them from a frozen stock?


I'm not sure, but i think i will be taking the bacteria from colonies already growing in a plate.
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-Arnlan

connief
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Re: Bacteria Transformation Efficiency

Postby connief » Sun Nov 16, 2014 8:27 pm

Well, if you are going to be doing these experiments in a university lab, then you can always ask people in the lab what the best way is to prep your bacteria for transformation as per the procedures for this project.

arnlan
Posts: 80
Joined: Sun Nov 24, 2013 12:05 pm
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Re: Bacteria Transformation Efficiency

Postby arnlan » Sun Nov 16, 2014 8:54 pm

Alright, sounds good.

If i have any questions prior to thursday, i'll ask.

Good Night
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-Arnlan

arnlan
Posts: 80
Joined: Sun Nov 24, 2013 12:05 pm
Occupation: Student: 8th grade

Re: Bacteria Transformation Efficiency

Postby arnlan » Sun Nov 16, 2014 8:56 pm

I actually have one more question. Is this project perfect for my level as an 8th grader? Is it below, average, or above my level? I would like to hear your inputs.
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-Arnlan

arnlan
Posts: 80
Joined: Sun Nov 24, 2013 12:05 pm
Occupation: Student: 8th grade

Re: Bacteria Transformation Efficiency

Postby arnlan » Tue Nov 18, 2014 5:26 am

I would also like help finding a data table, where i will plot and put all my data. Is there one already, or will i have to make one.?
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-Arnlan

connief
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Re: Bacteria Transformation Efficiency

Postby connief » Sat Nov 22, 2014 2:29 pm

Hi,

I think this project is good for your level. The most important thing is that you're interested in the question that you're asking and that you understand what the experiment is testing and what your results tell you. As for a data table, I would first gather all your data and then we can figure out what is the best way to present it. If you are working with people in a university lab, you can always ask them for advice on what is the best way to present your results as well.

Connie

arnlan
Posts: 80
Joined: Sun Nov 24, 2013 12:05 pm
Occupation: Student: 8th grade

Re: Bacteria Transformation Efficiency

Postby arnlan » Sat Nov 22, 2014 3:35 pm

Hello,

I started working on the project on friday!!

It's working out well. Today we plated the ecoli on the 2 plates, and now we're waiting for them to grow. We just put them in the incubator.
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-Arnlan


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