A quick question about observation

Ask questions about projects relating to: biology, biochemistry, genomics, microbiology, molecular biology, pharmacology/toxicology, zoology, human behavior, archeology, anthropology, political science, sociology, geology, environmental science, oceanography, seismology, weather, or atmosphere.

Moderators: AmyCowen, kgudger, MadelineB, Moderators

Locked
belladaisy2018
Posts: 1
Joined: Mon Apr 16, 2018 1:08 am
Occupation: Student

A quick question about observation

Post by belladaisy2018 »

im wondering what other things i can observe instead of zone of inhibitation in agar plate,...

Any observation i can observe from it? uhm any thing to see?

by the way, im trying to get a research on making an ointment antibacterial one made from Star apple leaves oil to kill Streptococcus Pyogenes...

Thank you for further help...
[link removed by moderator]
iriss
Former Expert
Posts: 11
Joined: Tue May 01, 2018 1:49 pm
Occupation: Student

Re: A quick question about observation

Post by iriss »

Hi,

It sounds like you are trying to figure out how to determine if an antibiotic ointment made from Star apple leaves oil will effectively kill streptococcus pyogenes bacteria. If so, testing the zone of inhibition on an agar plate is a great place to start!

The main observation you should look for is the diameter of the zone of inhibition (measuring with a ruler should be fine). This tells you the effectiveness of your antibiotic ointment. You can determine the effectiveness by comparing your zone size to a standardized chart.

There are a number of factors that can affect the zone of inhibition, such as the density of the bacteria on the agar plate, the ambient temperature, moisture levels, how much of the antibiotic you use, and what specific components you use in your agar. That means you’ll need to control for all these factors and make note of their values when you run your test.

For more detailed information about the zone of inhibition and how to interpret your results, try this link: https://biologywise.com/what-does-zone- ... ition-mean

If you want to make other observations during your experiment, you could run your test multiple times while purposefully altering one condition (how much antibiotic you use, for example) to see how it affects your zone of inhibition. You might also consider timing your experiment to see how long it takes for your ointment to spread/bacteria to grow. Then ask yourself: Why does time matter? Are there any conditions you could alter that would speed up or slow down this process?

If you’re looking for other ways to test the effectiveness of your antibiotic besides using an agar plate here is a helpful link: https://amrls.cvm.msu.edu/microbiology/ ... ng-methods

Note: for many of these experiments you will need additional equipment, such as test tubes and an electronic testing strip.

I hope that helps!
Locked

Return to “Grades 9-12: Life, Earth, and Social Sciences”