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Growing Yeast and Bacteria

Postby aanikak » Sat Jul 28, 2018 1:40 am

I'm in the early stages of designing an experiment, but I wanted to test the effect of adding a probiotic bacteria (Lactobacillus acidophilus) to a malt agar plate with a yeast (candida albicans). I would measure zones of inhibition after adding an anti fungal medication, but only wanted to quantify the growth of the yeast rather than the probiotic bacteria. Is there any way to differentiate between the two while counting colonies? thanks

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Re: Growing Yeast and Bacteria

Postby AkaAlex » Mon Jul 30, 2018 7:34 am

Hi, thanks for your question.

In order to differentiate between yeast colonies and bacterial ones, the most accurate method would be to use a microscope. If one is not available, it is possible to roughly differentiate between the colonies based on morphological differences. Yeast is often more cloudy and creamy, while bacteria can appear much more varied in color and shape.

Interpreting plates can be difficult, but the following link should help you get started:

https://www.sciencebuddies.org/science- ... gar-plates

Good luck on your experiment :D

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