I am currently doing a project where I will treat bacterial (E. Coli) biofilms with a colloidal silver solution. I was wondering what the best was to grow and treat the biofilms with the solution.
Bacteria:
https://www.carolina.com/bacteriophage- ... ion=e+coli
Solution:
https://www.amazon.com/Natural-Path-Sil ... B000BT4CFE
Biofilms
Moderators: AmyCowen, kgudger, MadelineB, Moderators
-
Life_Science_Guidance
- Posts: 1
- Joined: Tue Feb 05, 2019 5:06 am
- Occupation: Teacher
Re: Biofilms
Ideally, you'd want to inoculate E-coli along with beads with the goal of forming biofilms on the bead surface and treat it with varying doses of your drug (colloidal silver).
A less perfect but simpler experiment would be to grow the bacteria on an agar plate, soak varying doses of your drug in filter paper discs, place them on the agar plate over 24-48 hours and measure the area in which the bacteria failed to grow. Highest bacteria free area is the best dose for inhibition. Note this measures bacterial growth inhibition, not necessarily biofilm production.
A less perfect but simpler experiment would be to grow the bacteria on an agar plate, soak varying doses of your drug in filter paper discs, place them on the agar plate over 24-48 hours and measure the area in which the bacteria failed to grow. Highest bacteria free area is the best dose for inhibition. Note this measures bacterial growth inhibition, not necessarily biofilm production.