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Albumin pH

Posted: Mon Feb 11, 2019 5:58 pm
by Miichawl
Are there any simple, cheap solutions that can be used to alter albumin ph levels? I will be using egg white as my source of albumin.

Re: Albumin pH

Posted: Tue Feb 12, 2019 11:55 am
by 17eugenekim
Vinegar is a commonly used acid; the main component is acetic acid and a solution of vinegar should have a pH of around 4~5, if I'm not mistaken. You could also use lemon juice or other citrus juices. This will help lower the pH.

To raise the pH, baking soda is another common substance; the chemical compound is sodium bicarbonate. Most soaps and cleaning materials act as bases, including bleach (of course, be careful when handling).

Whatever you do, it would be helpful to use pH strip paper to confirm what you've done to your albumin. Hope that helps!

Albumin Denaturation

Posted: Mon Apr 15, 2019 10:14 am
by Miichawl
Hi, I know this a very specific question, but does anyone know if albumin, which has a pH level of 7.4 would denature faster from pH changes if it was more alkaline or acidic?