Shipon wrote:Dear replier,
Thank you very much. In our country this work is very relevant and very improtant. so i prefer this tophic. Here I like to mention that you said that this work is very easy. But in litearatue survey I found many papers that Hydrogen peroxide has an effect for seed germination. There are not detailed information. I design my experiment in my own way. Just I liked to collect some additional information from you.So if there is any information please infrom me.
I totally agree with MelissaB, this project is inappropriate for a Ph.D. student, and this forum is not the appropriate site for you to be asking for help. To me, it is horrifying that you are even asking for help here, especially if you already have two college degrees.
You asked three questions in your first post:
1)Now I want to know that If there is any information that previously this experiment was done, what experimental design I will take?
The experimental provided on this site is rated "6", which is for a 12 year old. I certainly would not use this as a basis for a Ph.D. project.
As a professional scientist, I can tell you experimental design is the hardest part of science. Once you have come up with a clever experiment, collecting the data is just a technical matter (not that it isn't hard). This is really what you are earning your Ph.D. for- designing experiments to test relevant hypothesis at a fairly sophisticated level. Then, redesign experiments, when the first ones don't work.
You should come up with a hypothesis with your thesis advisor. And, "Hydrogen peroxide inhibits root cutting growth" is, again, a hypothesis for a 12 year old. Now, something like, "Hydrogen peroxide inhibits growth through destruction of the blah, blah enzyme, inhibiting the blah mechanism" (where "blah" is just a filler. I haven't done the research, so I can't even begin to tell you how hydrogen peroxide does work on plants.) Then you could do some sophisicated experiment on the enzyme and its mechanicism. As a chemist, I doubt that hydrogen peroxide is a good chemical to use. My bet is that it just chews up everything and there aren't any interesting studies. But, do some reading.
2) What is the Outcomes of this Project?
No idea. We help students do there own project following the scientific method. We help the children work up whatever data they collect, and reconcile it with their hypotheses. This isn't a peer reviewed journal, and we don't have an answer key for results (but who does, really?)
3) And Some references relating with this projcet.
You said you have already done literature searches. Do some more. We don't provide references to peer reviewed journals. First, most people don't have access and secondly, these papers are too complicated for children. If you can't find anything in the literature, maybe that is because this project is too simple.
Lastly, you really need to talk to your thesis advisor. You need a complicated, multipart project that will take a few years to complete, and which should lead to multiple papers and is on a topic your advisor can assist you with.