1)I'm planning on diluting ethanol to 70% with water to later use for making stock solutions. What kind of water should I use? Is it okay to use tap water?
2) My sample sizes will be 50 ml of algae culture and 200 ml of culture media. To achieve the final concentration of phytohormones of 10.0 mg/ml, how should I make the stock solution and how much should I add to the algae culture? (I only have 100 mg of phytohormones). For your reference, here is a procedure I found online, but it's unclear and confusing.
3) What is the difference between mg/ml and mg/l?To prepare a 1 mg/L stock solution: Add 100 mg of the plant growth regulator to a 100 mL volumetric flask or other glass container. Add 2-5 mL of solvent to dissolve the powder. Once completely dissolved, bring to volume with double processed water (Product No. W3500). Stirring the solution while adding water may be required to keep the material in solution. Store the stock solution as recommended in the tables. Add 1.0 mL of the stock solution to 1 liter of medium to obtain a final concentration of 1.0 mg/L of the plant growth regulator in the culture medium
4) Finally, how do you perform the error percentage test and the chi-squared test for this kind of experiment?
Once again, thank you so much for your time and effort. Have a great day!