Science Buddies: "Ask an Expert"

Hi,

I am trying to do a similar project, but I am planning on extracting antioxidants from a type of algae called Dunaliella and observing its effects on yeast treated with different oxidants. I was just wondering how you induced the oxidants into the cancer tissue and how you measured the oxidative stress. I am planning to measure the effects with a hemocytometer.

Thank you

Hi, I did not induce the oxidants into the cancer cells. Cancer cells generate reactive oxygen species as a part of normal cellular functions; it also generates more ROS than normal cells due to it's increased activeness.

Also, I measured ROS generation using a flow cytometer with a fluorescent probe.