Science Buddies: "Ask an Expert"

Am having this project to carry out. The title of the project is Antimicrobial resistant pattern of E. coli and Staphylococcus aureus isolated from clinical samples, but am having a problem on how to prepare the local sensitivity disk. Please i need help, thanks.

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[Administrator note: Other post:]

am carrying my final year project on the topic: ANTIMICROBIAL RESISTANT PATTERN OF Esherichia coli and Staphylococcus aureus isolated from clinical samples. So am having problem with accessing relevant literature and how to prepare the sensitivity disk locally. Please i need your help on these. Thanks


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Hello:

Quite a currently relevant project, especially considering the rapid increase in antibiotic resistance strains of bacteria. The standard method to perform disk diffusion antibiotic sensitivity is the Kirby-Bauer method. If you Google this method you should come up with a lot of hits that describe the method. If you have access to the antibiotics that you want to test or if you want to look at sensitivity to household disinfectants, etc you can simply make little disks from filter paper with a paper hole punch and drop a known amount of solutions of a known concentration (or varying concentrations), let these dry and then use these as your test disks.

Goo luck and just post if you have further questions.

Bob

Thank you, for your assistant