Science Buddies: "Ask an Expert"

Hello!
I am a junior in high school researching how shelf life of supermarket chicken effects the formation of advanced glycation end products. I was wondering if anybody had a protocol on how to break down the meat into a solution in which I could perform an ELISA test on. Would I need some kind of buffer solution? I have read articles about using saline solution but have been unable to find a specific protocol. Any suggestions would be much appreciated.

Thank You,
Maya

Hello!

I am unfamiliar with this process unfortunately. However, there is a website that details how to prepare certain samples for an ELISA. http://www.abcam.com/protocols/elisa-sa ... on-guide-1.

If you already have an ELISA kit, you can actually call a representative from the company that you got the kit from, and ask them how to prepare samples as well. Sometimes different kits have different buffers they want you to use. This might be the best place to start. Hope this helps!

-Surya