Science Buddies: "Ask an Expert"

Hi, is there anyone who knows how to enumerate bacteria by using fluorescent images? Does anyone can explain this: an article reported RGB images (24bit), 512x512 pixels, a final pixel resolution is 0.2 um/pixel, a zoom factor is 2.0. Then the article said these images represented a volume of 1.05 x 10-8 ml per field of view, thus for direct enumeration of bacteria per milliliter, a microscopic factor of 1.05 x 108 was used. I don't know how it processed?

Hi DaisyMM,

I think your question is better suited for the Life, Earth, and Social Sciences forum. The experts there will be better able to help you. I'm asking a moderator to move your post so that the life sciences experts can help you.

Thanks Terik