Science Buddies: "Ask an Expert"

Hi I’m having trouble to devise an experiment to carry out this task: An insert is available containing a gene within it whose orientation and exact position with the insert are unknown. The gene contains a sequence for an important small peptide hormone. No DNA sequence info is available. Set up an experimental scheme that takes into account the task described above, beginning with the insert and the expression vector pUC18. A map of pUC18 with all features indicated, including the multi-cloning site, may be easily obtained by typing the vector name into Google. The insert containing the peptide gene is a blunt end fragment of 1.8kb that was not generated with a restriction enzyme. The gene does not have a promoter and there is 6* his tag at the C-terminus of the open reading frame.

Hello ctwz and welcome to Science Buddies,

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