Science Buddies: "Ask an Expert"

Hi there!
I am doing my science fair project on Lactobacillus. I am planning to supplement it with different vitamins and see if these supplements will strengthen the colonies enough for it to survive penicillin. (partially survive). I was wondering how I could grow the Lactobacillus using MRS agar. The Lactobacillus will be coming from capsules.

Thanks so much for the help!!

Hi EshanRasul,

Here is another thread post that addresses your question. If you have further questions let us know.

viewtopic.php?t=15667

Elizabeth

Hi Elizebeth thank you for the info
I was just wondering, that the Lactobacillus that I ordered comes in a capsule. So what kind of broth could I use to culture it in?
Once again thanks

Hi EshanRasul,

You grow Lactobacillus in a media that is the same composition as you would use to make up the agar plates (except dont add any agar).

https://www.bd.com/europe/regulatory/As ... 288110.pdf

Elizabeth

Hi Elizabeth,

Thank you for your reply I was just wondering that since my lactobacillus comes from a capsule would I just pour it onto the agar to grow it?

Thanks

Hi there I am very disappointed I posted my question about three weeks ago and haven't recieved a reply I really need to conduct my experiment so can someone please leave me an answer?

Hi EshanRasul,

I have never worked with these capsules so I do not have any first hand experience on the best way to culture them. I am sorry no one else has been able to get back to you. In the previous thread I posted earlier they mention that you can not just pour the powder on the plate and suggest diluting it in 0.9% sodium chloride solution - details are provided in the post. viewtopic.php?t=15667

Elizabeth