PLEASE HELP! Gel Electrophoresis used to compare Plant Pigments
Posted: Wed Mar 01, 2017 10:37 pm
Hi! So I just began working on my 10th grade science fair project with the question of, By using our own gel electrophoresis and observing the pigments molecular migration, can we conclude that flowers of the same visible color, but different species have a phylogenetic relationship? But, I have now realized some road bumps I don't know how to approach? i was planning on using the at home gel electrophoresis procedure found on science buddies but, I don't know exactly how i should create my test sample from the flower petals that I'd place in the gel?
i know about this “Use your gel electrophoresis chamber to determine if two different types of plants use the same molecule for pigment. To prepare your samples, take the flowers from a plant, grind up the flower, add a little bit of isopropyl alcohol, and continue grinding. Once the solids settle, pour the pigment-tinted alcohol into a separate container. Let most of the alcohol evaporate and then add a drop or two of your buffer solution to reconstitute the pigment.”, But, i don't know how much and then also, I found that anthocyanins aren't negatively charged and so I'd have to coat it with sodium dodecyl sulfate but I don't know how? Also, what is the buffer solution mentioned above I'm supposed to add?
I;m just confused as to the exact procedure I need to use to view the molecular migration of the anthocyanin present in about 5 different types of flower species, but all are the same shade of red?
PLEASE HELP!
i know about this “Use your gel electrophoresis chamber to determine if two different types of plants use the same molecule for pigment. To prepare your samples, take the flowers from a plant, grind up the flower, add a little bit of isopropyl alcohol, and continue grinding. Once the solids settle, pour the pigment-tinted alcohol into a separate container. Let most of the alcohol evaporate and then add a drop or two of your buffer solution to reconstitute the pigment.”, But, i don't know how much and then also, I found that anthocyanins aren't negatively charged and so I'd have to coat it with sodium dodecyl sulfate but I don't know how? Also, what is the buffer solution mentioned above I'm supposed to add?
I;m just confused as to the exact procedure I need to use to view the molecular migration of the anthocyanin present in about 5 different types of flower species, but all are the same shade of red?
PLEASE HELP!