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Physarum Polycephalum Spores???

Posted: Tue Mar 06, 2018 7:40 pm
by deleted-565887
So I decided to do the "Smarter Than Your Average Slime: Maze-solving by an Amoeboid Organism" and I already bought all the materials including the slime mold. A few days later, it started to form black things or I guess spores but now I need the slime to be active again since I don't have that much time to order another active culture of they Physarum.

Is there a way where I can reverse the spores and turn it back into the plasmodium?

Along with that is there a way I can also reverse the fruiting bodies back so it can become the plasmodium that was first given when I bought the mold??

Also if there was a way, how long would it take?

Thank you to whoever will read and reply! :D

Re: Physarum Polycephalum Spores???

Posted: Wed Mar 07, 2018 2:02 am
by deleted-547689
According to Ward's Scientific, you should be able o germinate the spores. These spores will germinate into haploid ameboid-like cells that will fuse together to form an actively growing diploid Plasmodium. I don't know how long this woud take though, and if you are short of time, this might not help. I copied the text below:
Life Cycle
The multiple nuclei in the macroplasmodium are diploid and all divide together (at the same time) without cytokinesis. This results in a multi-nucleate syncytium. Under adverse conditions, where temperature and moisture are not suitable for continued growth, the plasmodium is converted into a resting structure termed a sclerotium. Sclerotia are hardened masses of irregular form consisting of many minute cell-like components. Under favorable conditions, the sclerotium is reactivated (converted) to actively growing plasmodial stage.
Under conditions of starvation in sunlight, fruiting bodies can form that will release haploid spores. When the spores germinate under moist conditions, they will germinate as either amoeboid cells or as flagellates if they germinate in a liquid environment. Two haploid amoeboid cells and their nuclei can fuse to begin growth into the multinucleated (with diploid nuclei) plasmodium.