help with sciFAIR project!!!
Posted: Wed Jan 22, 2020 3:43 am
I am doing my experiment on the effects of caffeine on C. elegans and I have a couple of questions. Essentially I plan to place the worms in the middle of an agar test plate and place a solution with the drug on one side and place nothing on the other side. I also plan to pre-expose the worms to different concentrations of caffeine, and then observe their behavior towards the drug.
1. How can I divide the test plate such that I will be able to create these two "chambers"?
2. If I put the caffeine solution on the agar, how can I ensure that it won't be absorbed into the agar?
3. Is there a chance that the worms will move randomly as they are not aware of which side contains the drug and which does not? If so, how can I prevent this?
Thanks in advance!!!
1. How can I divide the test plate such that I will be able to create these two "chambers"?
2. If I put the caffeine solution on the agar, how can I ensure that it won't be absorbed into the agar?
3. Is there a chance that the worms will move randomly as they are not aware of which side contains the drug and which does not? If so, how can I prevent this?
Thanks in advance!!!