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I need help with crown gall ASAP!
Posted: Sat Jan 06, 2007 2:26 pm
by Tim Miller
Please help! I chose an experiement that requires diagnosing garlic to prevent crown gall in plants. I have the plants but I need to find out how to get the bacteria (Agrobacterium Faciens) desperately! I can't get a plant that's already infected! There's no way I'm buying one for 100 dollars! Why was this a science project topic of getting the bacteria is almost impossible? Is there anyway I can get chemicals from a science teacher or in my house to make/infect crown gall? If so, what are they? PLEEEEEEAASE HELLLLP!!!!
Posted: Sat Jan 06, 2007 2:35 pm
by Lise Byrd
Tim,
The page I found that describes your project suggests using an already-infected plant as the source of A. tumefaciens. Have you tried asking around at local nurseries? If one has a crown gall-infected plant, I see no reason why they wouldn't be willing to give it to you for under $50.
Hope this helps,
Sonia
what about making it?
Posted: Sat Jan 06, 2007 2:41 pm
by Tim Miller
Are you sure there's absolutely no chemicals found in a household or
HIGH SCHOOL LAB I could use???
Posted: Sat Jan 06, 2007 3:30 pm
by Lise Byrd
A. tumefaciens is a living organism, so you would not be able to use chemicals to make crown gall.
http://www.sciencestuff.com/ctgy/5Ls-LiveBac offers
A. tumefaciens for $13. I don't know how reliable the site is, however.
Sonia