Here is my recipe for my Agar Plates in case this is the problem:
Ingredients:
500 mL of Beef broth
15g of Agar
10g of sugar
50g-200g of non-iodized salt
Distilled H2O
Recipe:
- Assemble all the ingredients in the recipe
- In the glass container, put all the ingredients in
- On the glass container, loosely put aluminum foil over the container (enough to where steam can escape)
- Sterilize the solution by boiling it on a hot plate for at least 15 minutes. Boil it until the agar is completely dissolved, and the media is clear.
- While boiling, swirl the media often to prevent the media from burning
- Cool the media until it is just cool enough to handle, about 20–30 minutes. One should be able to comfortably hold their hand against the container for a few seconds. If the media is too cool, it will solidify in the container. If it is too hot, it will leave excess condensation on the lids.
- Remix the media before pouring. Be careful not to over mix to not add bubbles to the media
- Pour into the plate until it covers the bottom using heat-resistant gloves, approximately 25 mL
- Incubate at 20-25 °C for 2–3 days. Bacteria grow more successfully at lower temperatures.