Experiment with S. Aureus/Epidermis on Agar Plates
Posted: Sat Mar 03, 2007 10:54 am
Hello there,
I am conducting an experiment on growing skin bacteria. For about 3 days, I incubated my streaked plates (informally streaked, I just swabbed it in a predictable pattern with a sterile applicator) at 20C for 72 hours. I saw no growth so I took it and incubated it since 35 hours ago (it is still being incubated) at about 35 - 39C. however, I am seeing a LOAD of condensation on my petri lids. Quite a bit of moisture is collecting on the lids (of course I incubated them upside down). I am using standard nutrient agar and incubated my plates right after the 20C incubation period. Is this normal, or preventable? I plan to incubate my plates for about 24 more hours. Will my agar dry out?
Thanks!
I am conducting an experiment on growing skin bacteria. For about 3 days, I incubated my streaked plates (informally streaked, I just swabbed it in a predictable pattern with a sterile applicator) at 20C for 72 hours. I saw no growth so I took it and incubated it since 35 hours ago (it is still being incubated) at about 35 - 39C. however, I am seeing a LOAD of condensation on my petri lids. Quite a bit of moisture is collecting on the lids (of course I incubated them upside down). I am using standard nutrient agar and incubated my plates right after the 20C incubation period. Is this normal, or preventable? I plan to incubate my plates for about 24 more hours. Will my agar dry out?
Thanks!