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Fluorometer
Posted: Fri Sep 19, 2008 2:21 am
by hpark4
We are looking for algae's chlorophyll measurement tool. Where can we rent or buy low cost fluorometer to measure the chlorophyll level?
If the cost of fluorometer is high, what will be the best way and easy way to measure chlorophyll level?
Re: Fluorometer
Posted: Sat Sep 20, 2008 11:46 pm
by barretttomlinson
Hi,
Here are a few resources for measuring chlorophyl, mostly by using a spectrophotometer
http://employees.csbsju.edu/SSAUPE/biol ... _quant.htm
http://www.chebucto.ns.ca/ccn/info/Scie ... hotometric
http://dipin.kent.edu/chlorophyll.htm
http://www.ecy.wa.gov/programs/wq/plant ... phyll.html
http://journals.tubitak.gov.tr/botany/i ... -96040.pdf
http://waterontheweb.org/curricula/ws/u ... 5-2003.pdf
Here is an EPA method for measuring chlorophyl by Flourescence;
http://www.epa.gov/nerlcwww/m445_0.pdf
And other resources using flourescence:
http://www.turnerdesigns.com/t2/doc/app ... _5101.html
Here is the first page of an article that looks relevant
http://www.springerlink.com/content/kl04qht7w415c02u/
I believe that you will find that buying or renting either a spectrophotometer or a fluorometer is way too expensive for a science fair project( cost is likwly more than $10000 for most instruments). I think you will find spectrophotometers are much easier to access than fluorometers. Most colleges have spectrophotometers in their laboratories. In your shoes, I would try to develop a relationship with a chemist in your local area and try to see if he/she can help you get access to an instrument for long enough to analyze your samples. Alternatively you might approach a manufacturer of the spectrometer or flourometer of your choice. Sometimes they have application laboratories with application chemists or sales people who might be willing to help you run a few samples on a demonstration basis. If you attempt either of these routes you must be prepared to be super organized with your samples and procedure well defined and ready, as if you can get access at all, it will likely be a one time thing for an hour or two.
I wish you every success with your project. It sounds like a fun undertaking!
Barrett Tomlinson
Re: Fluorometer
Posted: Sun Sep 21, 2008 6:19 am
by hpark4
Thanks a lot for the information. Your information helps this project greatly. Thanks again.
Re: Fluorometer
Posted: Wed Sep 24, 2008 8:48 am
by stanthescienceman
wat up yo