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Harvesting Plasmid DNA from Bacterial Lysate

Posted: Fri Nov 13, 2009 10:59 am
by juman
Hi,

My project is due on November 15, 2009.
Please reply to my questions.
Thank you.

Where is plasmid DNA found in the cell?
Why do some people sequence plasmid DNA rather than chromosomal DNA?
Is bacterial lysate produced when lysis occurs to a bacteria cell?

Re: Harvesting Plasmid DNA from Bacterial Lysate

Posted: Mon Nov 16, 2009 9:51 am
by donnahardy2
Hi,

I'm sorry we did not have a volunteer available to answer your questions before the deadline. Is this for a science fair project? Are you going to try to purify plasmids? The purpose of the science buddies website is to answer questions about researching and conducting science fair projects, and this topic could make an excellent project. A plasmid is a cirHere is some background information that will help answer your questions:

http://www.wfcc.info/tis/info12.html

http://ricfacility.byu.edu/Plasmid%20DN ... lation.pdf

http://en.wikipedia.org/wiki/Plasmid

Good luck!

Donna Hardy

Re: Harvesting Plasmid DNA from Bacterial Lysate

Posted: Thu Nov 19, 2009 8:56 am
by juman
thank you.