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Chromatography

Posted: Thu Oct 14, 2010 1:04 am
by EyeAmLoz
Can anyone tell me if the temperature of the solvent used in chromatography will affect the Rf value?
thankee :)

oh, if you have a source such as a previous experiemnt that would be amazing. :)

Re: Chromatography

Posted: Thu Oct 14, 2010 9:36 pm
by deleted-71712
This page lists some factors that influence Rf values, and temperature is among them:

http://orgchem.colorado.edu/hndbksupport/TLC/TLCrf.html

I put "solvent temperature retention factor" into a search engine, and many of the results were studies of the effect of temperature on Rf in various specific systems. If you want to search specifically for scientific papers, here is some useful advice:

https://www.sciencebuddies.org/science- ... pers.shtml

Amanda