|Time Required||Long (2-4 weeks)|
|Prerequisites||Should have basic knowledge of chemistry and chemical reactions.|
|Material Availability||Readily available|
|Cost||Low ($20 - $50)|
|Safety||Use caution when choosing a biochemical reaction to study. For more information about chemical safety, consult the Chemistry Safety Guide.|
AbstractMany proteins act as catalysts which speed up or regulate a biochemical reaction. The kinetics of an enzyme can be investigated by measuring the reactants and products of the reaction over time. Research the enzymatic reaction of a protein and investigate its kinetics. Measure the production of hydrogen peroxide (H2O2) by catalase, the digestion of gelatin by papain, or any other enzymatic reaction you have access to. Bio-Rad's Biofuel Enzyme Kit provides a great measurement platform for exploring cellobiase's role in catalyzing a reaction to produce cellulosic ethanol, a type of biofuel, but you will need a teacher's help to order the kit as Bio-Rad only sells to schools; see this page for details on purchasing from Bio-Rad. Once you have a method for measuring the kinetics of the reaction, conduct a series of experiments to measure the enzyme kinetics under different conditions. For example, you could systematically change the concentration of dissolved salts, or the temperature, or the pH of the solution in which you are measuring the enzyme kinetics. Or you could add an excess of one or more reactants, or an excess of one or more products to see what effect this would have on the enzyme kinetics.
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Last edit date: 2017-07-28
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