Reference Materials

Microbiology Techniques & Troubleshooting

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The following Microbiology resources were developed by the Department of Biology at the University of Missouri in St. Louis and are reproduced, without modification, here with their permission. The originals can be found at: http://www.umsl.edu/~microbes/techniques.html

Microorganisms Safety Guide	A Science Buddies resource
Storing Bacterial Strains	How to store a bacterial strain for later use
Culture Problems	Descriptions of some common problems and solutions to those problems
Sterile Technique	Methods for sterile transfer of cultures in broth or on plates
Optimum Temperatures	Optimum temperatures for growth of common lab strains
Sterilizing	Methods for sterilizing media and glassware in the classroom
Streaking plates	Method for streaking plates to obtain isolated colonies
Nutrient Agar	Instructions for preparing nutrient broth, plates, and slants
Tips for Pouring and Storing Agar Plates	Tips for pouring and storing plates
Incubator	A low-cost incubator for the classroom
Homemade Media	An inexpensive alternative to commercial media
Testing Autoclaves	Commercial spore solutions test sterilizers
Tyndallization	An old-fashioned method for sterilizing broth
Simple Staining of Microbes	Instructions for staining bacteria

Source

University of Missouri, St. Louis Department of Biology. (n.d.). Techniques and Troubleshooting. Science in the Real World: Microbes in Action Program. Retrieved June 30, 2010 from http://www.umsl.edu/~microbes/techniques.html

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