What is cloning and how is it used in the biotechnology industry? How are plasmids constructed for use in a bacterial expression system?
You can investigate bacterial expression with the from the
Bio-Rad Biotechnology Explorer Program to make bacteria express green fluorescent protein (GFP), as shown in Figure 1, below
(see the Science Buddies project,
With variations to this kit you can test the conditions for pGLO expression under different conditions. You can also combine with…
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Average (6-10 days)
Some laboratory experience is required: knowledge of sterile technique, working with bacterial cultures, and using automatic pipettes are all helpful.
This science project requires the purchase of Bio-Rad's . Details about how to order products from Bio-Rad can be found .
Very High (over $150)
Adult supervision is required in the laboratory facility. Use sterile technique. Read the before starting any experiments. may be necessary.
Many continents contain large mountain ranges that divide the continent into different regions. In the U.S. the Rocky Mountains mark the continental divide. The presence of a large mountain range can have a big effect on seasonal weather patterns. Also, the weather and climate on one side of a mountain range may be very different from weather and climate on the other side of the range. In the case of the Rocky Mountains, the western slope and eastern slope each have very different climates…
What are cold fronts and warm fronts? What happens when a cold front meets a warm front? You can test this using different temperatures of water. Use food coloring to label the cold and hot water, then carefully combine the two liquids together. What happens? How do they mix? You can also fill water balloons with hot or cold water, and then float in a hot or cold water bath. How does temperature affect the movements of the balloon? You can do similar experiments with hot and cold air in…
How does temperature change as barometric pressure changes? You can make a device to test this using a barometer and a thermometer on your stovetop. You can collect your own weather data from a barometer and thermometer over a period of a week or month. You can also use data from a weather station to plot the relationship between barometric pressure and temperature. Does the pressure change as humidity changes? Measure the pressure in a humid and non-humid environment (like your bathroom…
As you move up or down in altitude or elevation, the temperature and pressure will change. This is particularly striking if you live near a mountain range. During the summer, at low altitudes you may have temperatures in the 80's or 90's and still be able to see snow on mountain peaks at high altitude. You can test the effect of altitude by comparing temperature data from weather stations at high and low altitudes. You can test the effect of elevation by making your own weather balloon and…
Ocean currents have a huge impact on our weather. If not for the ocean currents, the global climate would be similar to an ice age. Do you live near the coast? Find out which currents are near your coastline. How do they affect your climate? Where do they come from? Do they bring colder or warmer water to your area? Are they seasonal? What do you think your area would be like without them? Every three to seven years there is a weather phenomenon called El Niño, which is caused by…
Does the force of drag have an effect on the distance the puck will travel? Think of a way to launch the puck with a reproducible force, and examine the effect of launching the puck in different orientations on the distance it travels. For more information on the physics, see Haché, 2002.
Think of a way to launch the puck with a reproducible force, and examine the effect of launching the puck in different orientations on the distance it travels. For more information on the physics, see Haché, 2002.
The same principles of geology that we use to investigate the Earth can also be applied to other planets. Visit the Astrogeology Research Program at the USGS to find out how information about the geology of other planets can be gathered (USGS, 2006). Can you make a map or model of another planet? What minerals are found on other planets? Which planets have similar composition to the earth? What kind of geological forces occur on other planets? Do other planets have earthquakes, landslides…
What is geomagnetism, and how does it affect the earth? Visit the USGS Geomagnetism program for more information about this invisible force (USGS, 2006). How is the earth's magnetic field patterned? Are the magnetic poles located at the exact North and South Pole? How can the fields be mapped on the Earth's surface? What is declination? Use the mapping tools to study changes in declination patterns over time. (USGS, 2006)
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