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Science Buddies Original
Science Fair Project Idea
Most people are not aware that the soil around them is a battle scene. The combatants are very small—bacteria on one side and bacteriophage on the other. The bacteriophage (or phage for short) try to pierce the outer coats of the bacteria and inject them with phage DNA. If successful, the DNA will take over the inner machinery of the bacterial cells and force them to make many copies of the phage. After the copies are made, the bacterial cells break apart, releasing new phage that start… Read more
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Time Required Long (2-4 weeks)
Prerequisites This is an advanced science project that requires access to a laboratory with a water bath and 37°C incubator. The kit you will use has enough supplies to perform the procedure one time. To repeat the procedure, you should order additional supplies (listed in the Materials and Equipment section), or make your own media and agar plates. Working with a science teacher or other mentor who is familiar with growing bacteria and titering phage would be helpful.
Material Availability You will need to order the bacteria, the phage, and the supplies from an online science supply store. See the Materials and Equipment list for details.
Cost High ($100 - $150)
Safety The bacteria (E. coli B) and the phage (T4r) are not dangerous, but you should follow common-sense safety guidelines and wear gloves and safety goggles when working in the lab. Adult supervision is recommended.
Free science fair projects.